6DBI

Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates

Summary for 6DBI

Related6DBI 6DBJ 6DBL 6DBO 6DBQ 6DBR 6DBU 6DBV 6DBW 6DBX
EMDB information7843 7844 7845 7846 7847 7848 7849 7850 7851 7852 7853
DescriptorRecombination activating gene 1 - MBP chimera, Recombination activating gene 2, Forward strand of 12-RSS signal end, ... (9 entities in total)
Functional Keywordsv(d)j recombination, synaptic rag complex, nicked rss intermediates, paired complex, recombination-dna complex, recombination/dna
Biological sourceEscherichia coli
More
Total number of polymer chains10
Total molecular weight449800.02
Authors
Wu, H.,Liao, M.,Ru, H.,Mi, W. (deposition date: 2018-05-03, release date: 2018-08-01, Last modification date: 2019-12-18)
Primary citation
Ru, H.,Mi, W.,Zhang, P.,Alt, F.W.,Schatz, D.G.,Liao, M.,Wu, H.
DNA melting initiates the RAG catalytic pathway.
Nat. Struct. Mol. Biol., 25:732-742, 2018
PubMed: 30061602 (PDB entries with the same primary citation)
DOI: 10.1038/s41594-018-0098-5
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers15 0.1% 2.7%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures
Download full validation report