6VTT

Cryo-EM Structure of CAP256-VRC26.25 Fab bound to HIV-1 Env trimer CAP256.wk34.c80 SOSIP.RnS2

Summary for 6VTT

• Entry DOI: 10.2210/pdb6vtt/pdb
• Related: 5DT1 6VRW
• EMDB information: 21372 21383
• Descriptor: Envelope glycoprotein gp120, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• Functional Keywords: v1v2, vrc26, cap256, hiv-1, sosip, vaccine, superinfecting, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1 (HIV-1); More
• Total number of polymer chains: 8
• Total formula weight: 284495.39

Authors

Gorman, J.,Kwong, P.D. (deposition date: 2020-02-13, release date: 2020-04-08, Last modification date: 2021-10-06)

Primary citation

Gorman, J.,Chuang, G.Y.,Lai, Y.T.,Shen, C.H.,Boyington, J.C.,Druz, A.,Geng, H.,Louder, M.K.,McKee, K.,Rawi, R.,Verardi, R.,Yang, Y.,Zhang, B.,Doria-Rose, N.A.,Lin, B.,Moore, P.L.,Morris, L.,Shapiro, L.,Mascola, J.R.,Kwong, P.D.
Structure of Super-Potent Antibody CAP256-VRC26.25 in Complex with HIV-1 Envelope Reveals a Combined Mode of Trimer-Apex Recognition.
Cell Rep, 31:107488-107488, 2020

PubMed Abstract: Antibodies targeting the V1V2 apex of the HIV-1 envelope (Env) trimer comprise one of the most commonly elicited categories of broadly neutralizing antibodies. Structures of these antibodies indicate diverse modes of Env recognition typified by antibodies of the PG9 class and the PGT145 class. The mode of recognition, however, has been unclear for the most potent of the V1V2 apex-targeting antibodies, CAP256-VRC26.25 (named for donor-lineage.clone and referred to hereafter as VRC26.25). Here, we determine the cryoelectron microscopy structure at 3.7 Å resolution of the antigen-binding fragment of VRC26.25 in complex with the Env trimer thought to have initiated the lineage. The 36-residue protruding loop of VRC26.25 displays recognition incorporating both strand-C interactions similar to the PG9 class and V1V2 apex insertion similar to the PGT145 class. Structural elements of separate antibody classes can thus intermingle to form a "combined" class, which in this case yields an antibody of extraordinary potency.

PubMed: 32268107
DOI: 10.1016/j.celrep.2020.03.052

Experimental method

ELECTRON MICROSCOPY (3.7 Å)