6KDJ

HIV-1 reverse transcriptase with Q151M/Y115F/F116Y:DNA:lamivudine 5'-triphosphate ternary complex

Summary for 6KDJ

• Entry DOI: 10.2210/pdb6kdj/pdb
• Related PRD ID: PRD_900003
• Descriptor: HIV-1 reverse transcriptase p66 subunit, HIV-1 RT p51 subunit, DNA/RNA (38-MER), ... (8 entities in total)
• Functional Keywords: lamivudine 5'-triphosphate, hiv-1, hbv, reverse transcriptase, drug resistance, drug sensitivity, transferase-dna complex, replication, transferase/dna
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 6
• Total formula weight: 257016.59

Authors

Yasutake, Y.,Hattori, S.I.,Tamura, N.,Maeda, K. (deposition date: 2019-07-02, release date: 2020-03-04, Last modification date: 2023-11-22)

Primary citation

Yasutake, Y.,Hattori, S.I.,Tamura, N.,Matsuda, K.,Kohgo, S.,Maeda, K.,Mitsuya, H.
Structural features in common of HBV and HIV-1 resistance against chirally-distinct nucleoside analogues entecavir and lamivudine.
Sci Rep, 10:3021-3021, 2020

PubMed Abstract: Chronic hepatitis B virus (HBV) infection is a major public health problem that affects millions of people worldwide. Nucleoside analogue reverse transcriptase (RT) inhibitors, such as entecavir (ETV) and lamivudine (3TC), serve as crucial anti-HBV drugs. However, structural studies of HBV RT have been hampered due to its unexpectedly poor solubility. Here, we show that human immunodeficiency virus type-1 (HIV-1) with HBV-associated amino acid substitutions Y115F/F116Y/Q151M in its RT (HIV) is highly susceptible to ETV and 3TC. Additionally, we experimentally simulated previously reported ETV/3TC resistance for HBV using HIV with F160M/M184V (L180M/M204V in HBV RT) substituted. We determined crystal structures for HIV-1 RT:DNA complexed with 3TC-triphosphate (3TC-TP)/ETV-triphosphate (ETV-TP)/dCTP/dGTP. These structures revealed an atypically tight binding conformation of 3TC-TP, where the Met184 side-chain is pushed away by the oxathiolane of 3TC-TP and exocyclic methylene of ETV-TP. Structural analysis of RT:DNA:3TC-TP also demonstrated that the loosely bound 3TC-TP is misaligned at the active site to prevent a steric clash with the side chain γ-methyl of Val184. These findings shed light on the common structural mechanism of HBV and HIV-1 resistance to 3TC and ETV and should aid in the design of new agents to overcome drug resistance to 3TC and ETV.

PubMed: 32080249
DOI: 10.1038/s41598-020-59775-w

Experimental method

X-RAY DIFFRACTION (2.51 Å)