Crystal structure of the human eIF4A1-ATP analog-RocA-polypurine RNA complex

Summary for 5ZC9

DescriptorEukaryotic initiation factor 4A-I, RNA (5'-R(*AP*GP*AP*GP*AP*GP*AP*GP*AP*G)-3'), PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (6 entities in total)
Functional Keywordsinitiation factor, dead-box, helicase, protein-rna complex, atpase, rocaglamide a, anticancer compound, translation, translation-rna complex, translation/rna
Biological sourceHomo sapiens (Human)
Total number of polymer chains2
Total molecular weight49264.61
Iwasaki, W.,Takahashi, M.,Sakamoto, A.,Iwasaki, S.,Ito, T. (deposition date: 2018-02-16, release date: 2019-01-16)
Primary citation
Iwasaki, S.,Iwasaki, W.,Takahashi, M.,Sakamoto, A.,Watanabe, C.,Shichino, Y.,Floor, S.N.,Fujiwara, K.,Mito, M.,Dodo, K.,Sodeoka, M.,Imataka, H.,Honma, T.,Fukuzawa, K.,Ito, T.,Ingolia, N.T.
The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA.
Mol. Cell, 2018
PubMed: 30595437 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2018.11.026
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.23030 0.3% 3.4% 0.71MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5zc9
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Molmil generated image of 5zc9
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Molmil generated image of 5zc9
rotated about y axis by 90°
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