5ZC9

Crystal structure of the human eIF4A1-ATP analog-RocA-polypurine RNA complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000339	molecular_function	RNA cap binding
A	0002183	biological_process	cytoplasmic translational initiation
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0003724	molecular_function	RNA helicase activity
A	0003725	molecular_function	double-stranded RNA binding
A	0003729	molecular_function	mRNA binding
A	0003743	molecular_function	translation initiation factor activity
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006413	biological_process	translational initiation
A	0008135	molecular_function	translation factor activity, RNA binding
A	0016020	cellular_component	membrane
A	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	30
Details	binding site for residue ANP A 500

Chain	Residue
A	PHE52
A	GLU183
A	GLY335
A	ASP337
A	GLN339
A	ARG362
A	ARG365
A	PHE366
A	MG501
A	HOH606
A	HOH613
A	LYS54
A	HOH618
A	HOH621
A	HOH626
A	HOH627
A	HOH629
A	HOH673
A	HOH702
A	HOH713
A	HOH726
A	HOH732
A	GLN59
A	HOH746
A	SER78
A	GLY79
A	THR80
A	GLY81
A	LYS82
A	THR83

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site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 501

Chain	Residue
A	ANP500
A	HOH613
A	HOH618
A	HOH629
A	HOH673

---

site_id	AC3
Number of Residues	10
Details	binding site for residue RCG B 600

Chain	Residue
A	PRO159
A	PHE163
A	GLN195
A	ASP198
B	A7
B	G8
B	HOH701
B	HOH702
B	HOH708
B	HOH724

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Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADEmL

Chain	Residue	Details
A	VAL180-LEU188

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00541

Chain	Residue	Details
A	ALA76

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site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS118
A	LYS174

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	THR158

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P60843

Chain	Residue	Details
A	LYS193

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P60843

Chain	Residue	Details
A	LYS238

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS146
A	LYS225
A	LYS309
A	LYS369
A	LYS381

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS238

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