5Z62

Structure of human cytochrome c oxidase

Summary for 5Z62

• Entry DOI: 10.2210/pdb5z62/pdb
• EMDB information: 6896
• Descriptor: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 7A2, mitochondrial, Cytochrome c oxidase subunit 7B, mitochondrial, ... (20 entities in total)
• Functional Keywords: cytochrome c oxidase, electron transport
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 14
• Total formula weight: 216219.87

Authors

Gu, J.,Zong, S.,Wu, M.,Yang, M. (deposition date: 2018-01-22, release date: 2019-02-27, Last modification date: 2025-04-09)

Primary citation

Zong, S.,Wu, M.,Gu, J.,Liu, T.,Guo, R.,Yang, M.
Structure of the intact 14-subunit human cytochrome c oxidase.
Cell Res., 28:1026-1034, 2018

PubMed Abstract: Respiration is one of the most basic features of living organisms, and the electron transport chain complexes are probably the most complicated protein system in mitochondria. Complex-IV is the terminal enzyme of the electron transport chain, existing either as randomly scattered complexes or as a component of supercomplexes. NDUFA4 was previously assumed as a subunit of complex-I, but recent biochemical data suggested it may be a subunit of complex-IV. However, no structural evidence supporting this notion was available till now. Here we obtained the 3.3 Å resolution structure of complex-IV derived from the human supercomplex IIIIIV and assigned the NDUFA4 subunit into complex-IV. Intriguingly, NDUFA4 lies exactly at the dimeric interface observed in previously reported crystal structures of complex-IV homodimer which would preclude complex-IV dimerization. Combining previous structural and biochemical data shown by us and other groups, we propose that the intact complex-IV is a monomer containing 14 subunits.

PubMed: 30030519
DOI: 10.1038/s41422-018-0071-1

Experimental method

ELECTRON MICROSCOPY (3.6 Å)