5VY4

Thermoplasma acidophilum 20S Proteasome using 200keV with image shift

Summary for 5VY4

Entry DOI	10.2210/pdb5vy4/pdb
EMDB information	8741 8742
Descriptor	Proteasome subunit alpha, Proteasome subunit beta (2 entities in total)
Functional Keywords	proteasome, hydrolase
Biological source	Thermoplasma acidophilum More
Total number of polymer chains	28
Total formula weight	658995.81
Authors	Herzik Jr., M.A.,Wu, M.,Lander, G.C. (deposition date: 2017-05-24, release date: 2017-06-14, Last modification date: 2024-03-13)
Primary citation	Herzik, M.A.,Wu, M.,Lander, G.C. Achieving better-than-3- angstrom resolution by single-particle cryo-EM at 200 keV. Nat. Methods, 14:1075-1078, 2017 Cited by PubMed Abstract: Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules. PubMed: 28991891 DOI: 10.1038/nmeth.4461 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.3 Å)

Structure validation