5VY4
Thermoplasma acidophilum 20S Proteasome using 200keV with image shift
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Number of Particle | 111184 |
| Software | FindEM,PHENIX,RELION,Leginon,CTFFIND,Gctf,Rosetta |
| Symmetry | D7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
