5VAJ

BhRNase H - amide-RNA/DNA complex

Summary for 5VAJ

• Entry DOI: 10.2210/pdb5vaj/pdb
• Descriptor: RNA (5'-R(*GP*AP*CP*AP*CP*CP*UP*GP*AP*(URU)P*C)-3'), DNA (5'-D(*GP*AP*AP*TP*CP*AP*GP*GP*TP*GP*TP*C)-3'), Ribonuclease H, ... (7 entities in total)
• Functional Keywords: amide-rna, sirna, rna x-ray structure, phosphate modification, ribonuclease h, amide modified rna-dna., hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Bacillus halodurans; More
• Total number of polymer chains: 4
• Total formula weight: 41197.95

Authors

Pallan, P.S.,Egli, M. (deposition date: 2017-03-27, release date: 2017-09-13, Last modification date: 2023-10-04)

• Primary citation: Mutisya, D.,Hardcastle, T.,Cheruiyot, S.K.,Pallan, P.S.,Kennedy, S.D.,Egli, M.,Kelley, M.L.,Smith, A.V.B.,Rozners, E.; Amide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs.; Nucleic Acids Res., 45:8142-8155, 2017; PubMed: 28854734; DOI: 10.1093/nar/gkx558

Experimental method

X-RAY DIFFRACTION (1.95 Å)