5UZ9

Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex

Summary for 5UZ9

• Entry DOI: 10.2210/pdb5uz9/pdb
• EMDB information: 8624
• Descriptor: CRISPR-associated protein Csy1, CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, ... (7 entities in total)
• Functional Keywords: crispr rna-recognition-motif (rrm), pseudo-helical, type 1-f crispr, immune system-rna complex, immune system/rna
• Biological source: Pseudomonas aeruginosa (strain UCBPP-PA14); More
• Total number of polymer chains: 13
• Total formula weight: 379215.91

Authors

Chowdhury, S.,Carter, J.,Rollins, M.F.,Jackson, R.N.,Hoffmann, C.,Nosaka, L.,Bondy-Denomy, J.,Maxwell, K.L.,Davidson, A.R.,Fischer, E.R.,Lander, G.C.,Wiedenheft, B. (deposition date: 2017-02-25, release date: 2017-04-26, Last modification date: 2024-03-13)

Primary citation

Chowdhury, S.,Carter, J.,Rollins, M.F.,Golden, S.M.,Jackson, R.N.,Hoffmann, C.,Nosaka, L.,Bondy-Denomy, J.,Maxwell, K.L.,Davidson, A.R.,Fischer, E.R.,Lander, G.C.,Wiedenheft, B.
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.
Cell, 169:47-57.e11, 2017

PubMed Abstract: Genetic conflict between viruses and their hosts drives evolution and genetic innovation. Prokaryotes evolved CRISPR-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-CRISPR (Acr) proteins that subvert these immune systems. The adaptive immune system in Pseudomonas aeruginosa (type I-F) relies on a 350 kDa CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease for target degradation. Here, we report the cryo-electron microscopy (cryo-EM) structure of the Csy complex bound to two different Acr proteins, AcrF1 and AcrF2, at an average resolution of 3.4 Å. The structure explains the molecular mechanism for immune system suppression, and structure-guided mutations show that the Acr proteins bind to residues essential for crRNA-mediated detection of DNA. Collectively, these data provide a snapshot of an ongoing molecular arms race between viral suppressors and the immune system they target.

PubMed: 28340349
DOI: 10.1016/j.cell.2017.03.012

Experimental method

ELECTRON MICROSCOPY (3.4 Å)