5UZ9
Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated protein Csy1 | polymer | 434 | 49194.2 | 1 | UniProt (Q02ML9) Pfam (PF09611) In PDB | Pseudomonas aeruginosa (strain UCBPP-PA14) | Cas8F |
2 | B | CRISPR-associated protein Csy2 | polymer | 327 | 36244.1 | 1 | UniProt (Q02MM0) Pfam (PF09614) In PDB | Pseudomonas aeruginosa (strain UCBPP-PA14) | Cas5F |
3 | C, D, E, F, G... | CRISPR-associated protein Csy3 | polymer | 341 | 37448.1 | 6 | UniProt (Q02MM1) Pfam (PF09615) In PDB | Pseudomonas aeruginosa (strain UCBPP-PA14) | Cas7F |
4 | I, J | Anti-CRISPR protein Acr30-35 | polymer | 77 | 8693.7 | 2 | UniProt (L7P7M1) Pfam (PF20829) In PDB | Pseudomonas phage JBD30 | AcrF1 |
5 | K | Anti-CRISPR protein 30 | polymer | 96 | 10760.5 | 1 | UniProt (Q6TM72) In PDB | Pseudomonas phage D3112 | Gene product 30, gp30, AcrF2 |
6 | L | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 189 | 21691.8 | 1 | UniProt (Q02MM2) Pfam (PF09618) In PDB | Pseudomonas aeruginosa (strain UCBPP-PA14) | Cas6F |
7 | M | CRISPR RNA (60-MER) | polymer | 60 | 19249.4 | 1 | GenBank (115583796) | Pseudomonas aeruginosa UCBPP-PA14 |
Sequence modifications
K: 8 - 96 (UniProt: Q6TM72)
L: 1 - 187 (UniProt: Q02MM2)
M: 1 - 60 (GenBank: 115583796)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
His 2 | - | expression tag |
His 3 | - | expression tag |
His 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Phe -1 | - | expression tag |
Thr 0 | - | expression tag |
Cys 22 | Ser 22 | see remark 999 |
PDB | External Database | Details |
---|---|---|
A 53 | G 2935992 | see remark 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 13 |
Total formula weight | 379215.9 | |
All* | Total formula weight | 379215.9 |