5UZ9

Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated protein Csy1	polymer	434	49194.2	1	UniProt (Q02ML9) Pfam (PF09611)	Pseudomonas aeruginosa (strain UCBPP-PA14)	Cas8F
2	B (B)	CRISPR-associated protein Csy2	polymer	327	36244.1	1	UniProt (Q02MM0) Pfam (PF09614)	Pseudomonas aeruginosa (strain UCBPP-PA14)	Cas5F
3	C, D, E, F, G... (C, D, E, F, G...)	CRISPR-associated protein Csy3	polymer	341	37448.1	6	UniProt (Q02MM1) Pfam (PF09615)	Pseudomonas aeruginosa (strain UCBPP-PA14)	Cas7F
4	I, J (I, J)	Anti-CRISPR protein Acr30-35	polymer	77	8693.7	2	UniProt (L7P7M1) Pfam (PF20829)	Pseudomonas phage JBD30	AcrF1
5	K (K)	Anti-CRISPR protein 30	polymer	96	10760.5	1	UniProt (Q6TM72)	Pseudomonas phage D3112	Gene product 30, gp30, AcrF2
6	L (L)	CRISPR-associated endonuclease Cas6/Csy4	polymer	189	21691.8	1	UniProt (Q02MM2) Pfam (PF09618)	Pseudomonas aeruginosa (strain UCBPP-PA14)	Cas6F
7	M (M)	CRISPR RNA (60-MER)	polymer	60	19249.4	1	GenBank (115583796)	Pseudomonas aeruginosa UCBPP-PA14

Sequence modifications

K: 8 - 96 (UniProt: Q6TM72)

PDB	External Database	Details
Met 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag

L: 1 - 187 (UniProt: Q02MM2)

PDB	External Database	Details
Phe -1	-	expression tag
Thr 0	-	expression tag
Cys 22	Ser 22	see remark 999

M: 1 - 60 (GenBank: 115583796)

PDB	External Database	Details
A 53	G 2935992	see remark 999

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	379215.9
All*	Total formula weight	379215.9

*Water molecules are not included.