5UZ9
Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 199348 |
| Software | FindEM,RELION,PHENIX,Leginon,UCSF Chimera,CTFFIND3,Coot |
| Symmetry | C1 |
| CTF correction | NONE |
