5UHC

Crystal structure of Mycobacterium tuberculosis transcription initiation complex containing 3nt RNA in complex with Rifampin

Summary for 5UHC

Related5UH6 5UH7 5UH8 5UH9 5UHA 5UHB 5UHD 5UHE 5UHF 5UHG
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (11 entities in total)
Functional Keywordsrna polymerase complex, transcription, dna, rna, transcription-dna-rna complex, transcription/dna/rna
Biological sourceMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Cellular locationCytoplasm  P9WGI1
Total number of polymer chains9
Total molecular weight436219.29
Authors
Lin, W.,Das, K.,Feng, Y.,Ebright, R.H. (deposition date: 2017-01-11, release date: 2017-04-12, Last modification date: 2017-11-22)
Primary citation
Lin, W.,Mandal, S.,Degen, D.,Liu, Y.,Ebright, Y.W.,Li, S.,Feng, Y.,Zhang, Y.,Mandal, S.,Jiang, Y.,Liu, S.,Gigliotti, M.,Talaue, M.,Connell, N.,Das, K.,Arnold, E.,Ebright, R.H.
Structural Basis of Mycobacterium tuberculosis Transcription and Transcription Inhibition.
Mol. Cell, 66:169-179.e8, 2017
PubMed: 28392175 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2017.03.001
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.796 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.26670.5%1.8%3.3%0.02MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5uhc
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Molmil generated image of 5uhc
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Molmil generated image of 5uhc
rotated about y axis by 90°