5U0S

Cryo-EM structure of the Mediator-RNAPII complex

Summary for 5U0S

Related5U0P
EMDB information8480 8479
DescriptorMediator complex subunit 6, Mediator complex subunit 8, Mediator complex subunit 17, ... (28 entities in total)
Functional Keywordstranscriptional, transcription, mediator, srb, rna polymerase ii, rna, pol2, activation, complex, transcription-transferase complex, transcription/transferase
Biological sourceSchizosaccharomyces pombe (Fission yeast)
Total number of polymer chains28
Total molecular weight953381.99
Authors
Tsai, K.-L.,Yu, X.,Gopalan, S.,Chao, T.-C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F. (deposition date: 2016-11-26, release date: 2017-03-08, Last modification date: 2018-07-18)
Primary citation
Tsai, K.L.,Yu, X.,Gopalan, S.,Chao, T.C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F.J.
Mediator structure and rearrangements required for holoenzyme formation.
Nature, 544:196-201, 2017
PubMed: 28241144 (PDB entries with the same primary citation)
DOI: 10.1038/nature21393
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers7611.1%7.9%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5u0s
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Molmil generated image of 5u0s
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Molmil generated image of 5u0s
rotated about y axis by 90°