5U0S
Cryo-EM structure of the Mediator-RNAPII complex
Summary for 5U0S
| Entry DOI | 10.2210/pdb5u0s/pdb |
| Related | 5U0P |
| EMDB information | 8479 8480 |
| Descriptor | Mediator complex subunit 6, Mediator complex subunit 7, Mediator complex subunit 21, ... (28 entities in total) |
| Functional Keywords | transcriptional, transcription, mediator, srb, rna polymerase ii, rna, pol2, activation, complex, transcription-transferase complex, transcription/transferase |
| Biological source | Schizosaccharomyces pombe (Fission yeast) More |
| Total number of polymer chains | 28 |
| Total formula weight | 953381.99 |
| Authors | Tsai, K.-L.,Yu, X.,Gopalan, S.,Chao, T.-C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F. (deposition date: 2016-11-26, release date: 2017-03-08, Last modification date: 2020-01-01) |
| Primary citation | Tsai, K.L.,Yu, X.,Gopalan, S.,Chao, T.C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F.J. Mediator structure and rearrangements required for holoenzyme formation. Nature, 544:196-201, 2017 Cited by PubMed Abstract: The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals. PubMed: 28241144DOI: 10.1038/nature21393 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.8 Å) |
Structure validation
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