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- EMDB-8480: Cryo-EM map of the Mediator-RNAPII complex -

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基本情報

登録情報
データベース: EMDB / ID: EMD-8480
タイトルCryo-EM map of the Mediator-RNAPII complex
マップデータMediator-RNAPII complex
試料
  • 複合体: complex of Mediator and RNAPII
    • タンパク質・ペプチド: x 28種
キーワードtranscriptional / transcription / Mediator / Srb / RNA polymerase II / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex
機能・相同性
機能・相同性情報


RNA Polymerase I Transcription Initiation / RNA polymerase II, holoenzyme / RNA polymerase II transcribes snRNA genes / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter ...RNA Polymerase I Transcription Initiation / RNA polymerase II, holoenzyme / RNA polymerase II transcribes snRNA genes / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / mRNA Capping / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / Estrogen-dependent gene expression / RNA Polymerase I Promoter Escape / mRNA Splicing - Major Pathway / Transcriptional regulation by small RNAs / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / regulation of septum digestion after cytokinesis / co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway / siRNA-mediated pericentric heterochromatin formation / core mediator complex / mediator complex / DNA-templated transcription elongation / intracellular phosphate ion homeostasis / chromatin-protein adaptor activity / termination of RNA polymerase II transcription / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / transcription by RNA polymerase III / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / positive regulation of translational initiation / nuclear-transcribed mRNA catabolic process / 7-methylguanosine mRNA capping / positive regulation of transcription initiation by RNA polymerase II / pericentric heterochromatin / RNA polymerase I complex / RNA polymerase III complex / transcription elongation by RNA polymerase I / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / transcription-coupled nucleotide-excision repair / translation initiation factor binding / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / P-body / transcription elongation by RNA polymerase II / euchromatin / mRNA transcription by RNA polymerase II / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / single-stranded DNA binding / transcription by RNA polymerase II / nucleic acid binding / transcription coactivator activity / protein dimerization activity / single-stranded RNA binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / nucleotide binding / regulation of transcription by RNA polymerase II / chromatin / nucleolus / DNA binding / RNA binding / zinc ion binding / metal ion binding / nucleus / cytoplasm / cytosol
類似検索 - 分子機能
Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like ...Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / Rpb4/RPC9 superfamily / RNA polymerase RBP11 / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerase subunit Rpb7-like / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / HRDC-like superfamily / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / : / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase subunit CX / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo11 / RNA polymerases L / 13 to 16 Kd subunits signature. / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site
類似検索 - ドメイン・相同性
DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Uncharacterized protein C24C9.04 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / DNA-directed RNA polymerase II subunit rpb7 / Mediator of RNA polymerase II transcription subunit 7 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit rpb4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 8 ...DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Uncharacterized protein C24C9.04 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / DNA-directed RNA polymerase II subunit rpb7 / Mediator of RNA polymerase II transcription subunit 7 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit rpb4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 8 / DNA-directed RNA polymerase II subunit rpb1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase II subunit RPB11 / Mediator of RNA polymerase II transcription subunit 17 / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 27 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 31
類似検索 - 構成要素
生物種Schizosaccharomyces pombe (分裂酵母)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.8 Å
データ登録者Tsai K-L / Yu X
資金援助 米国, 2件
OrganizationGrant number国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM67167 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM41628 米国
引用ジャーナル: Nature / 年: 2017
タイトル: Mediator structure and rearrangements required for holoenzyme formation.
著者: Kuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias /
要旨: The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is ...The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.
履歴
登録2016年11月26日-
ヘッダ(付随情報) 公開2017年3月8日-
マップ公開2017年3月8日-
更新2024年11月20日-
現状2024年11月20日処理サイト: RCSB / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.08
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.08
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-5u0s
  • 表面レベル: 0.08
  • UCSF Chimeraによる作画
  • ダウンロード
  • 単純化した表面モデル + あてはめた原子モデル
  • 原子モデルPDB-5u0s
  • Jmolによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_8480.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_8480.map.gz / 形式: CCP4 / 大きさ: 20.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Mediator-RNAPII complex
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
2.62 Å/pix.
x 176 pix.
= 461.12 Å		2.62 Å/pix.
x 176 pix.
= 461.12 Å		2.62 Å/pix.
x 176 pix.
= 461.12 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 2.62 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベルBy EMDB: 0.08 / ムービー #1: 0.08
最小 - 最大-0.11419473 - 0.22855034
平均 (標準偏差)-0.000103825936 (±0.014926006)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ176176176
Spacing176176176
セルA=B=C: 461.12 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z2.622.622.62
M x/y/z176176176
origin x/y/z0.0000.0000.000
length x/y/z461.120461.120461.120
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS176176176
D min/max/mean-0.1140.229-0.000

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添付データ

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試料の構成要素

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全体 : complex of Mediator and RNAPII

全体名称: complex of Mediator and RNAPII
要素
  • 複合体: complex of Mediator and RNAPII
    • タンパク質・ペプチド: Mediator complex subunit 6
    • タンパク質・ペプチド: Mediator complex subunit 8
    • タンパク質・ペプチド: Mediator complex subunit 17
    • タンパク質・ペプチド: Mediator complex subunit 18
    • タンパク質・ペプチド: Mediator complex subunit 20
    • タンパク質・ペプチド: Mediator complex subunit 11
    • タンパク質・ペプチド: Mediator complex subunit 22
    • タンパク質・ペプチド: Mediator complex subunit 14
    • タンパク質・ペプチド: Mediator complex subunit 4
    • タンパク質・ペプチド: Mediator complex subunit 7
    • タンパク質・ペプチド: Mediator complex subunit 21
    • タンパク質・ペプチド: Mediator complex subunit 31
    • タンパク質・ペプチド: Mediator complex subunit 27
    • タンパク質・ペプチド: Mediator complex subunit 9
    • タンパク質・ペプチド: Mediator complex subunit 19
    • タンパク質・ペプチド: Mediator complex subunit 10
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb1
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb2
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb3
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb4
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb5
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb6
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb7
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb8
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb9
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb10
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb11
    • タンパク質・ペプチド: RNA polymerase II subunit Rpb12

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超分子 #1: complex of Mediator and RNAPII

超分子名称: complex of Mediator and RNAPII / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 1.5 MDa

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分子 #1: Mediator complex subunit 6

分子名称: Mediator complex subunit 6 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 24.928947 KDa
配列文字列: MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLW VIQKQNRLNE NEVKPLTVYF VCNENIYMAP NAYTLLATRM LNATYCFQKA LTKIEKFPQY NPQEGYTYPK L SNDNLEVD ...文字列:
MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLW VIQKQNRLNE NEVKPLTVYF VCNENIYMAP NAYTLLATRM LNATYCFQKA LTKIEKFPQY NPQEGYTYPK L SNDNLEVD HSNTNEPADE NKNQSIENAD YSFSPEDFSV VRAFMQSLHS SKEAPDVK

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

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分子 #2: Mediator complex subunit 8

分子名称: Mediator complex subunit 8 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 23.360068 KDa
配列文字列: MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL SNNLAAHSHT LQTTSIYPSL EFPVKEQEP LLTTLLRTKA LPEVEEWEAN TLQEYEASIS SQPKKKEAND AYQKDQLWDQ ARIIFMEERE NYSWFDFVTR R QESEGEFV ...文字列:
MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL SNNLAAHSHT LQTTSIYPSL EFPVKEQEP LLTTLLRTKA LPEVEEWEAN TLQEYEASIS SQPKKKEAND AYQKDQLWDQ ARIIFMEERE NYSWFDFVTR R QESEGEFV SQRQLEIDRA TEEQNANQML TDILSFMKSG KR

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

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分子 #3: Mediator complex subunit 17

分子名称: Mediator complex subunit 17 / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 62.55141 KDa
配列文字列: MAEEANKDAD ISSLSLSLDP EIIGGQNNFL ENNLQQIFQK IIQERGPFRD LKEEDLQKEL QKESIKDESS AKSSETENVL EFATLDSKR NVNDTEVESM DSQAYKKELI EQIMIAQTEC SLALDMTSLL LSKFKENSIE TISPFLKSTV PPSSLQFSRS Q PPESKESD ...文字列:
MAEEANKDAD ISSLSLSLDP EIIGGQNNFL ENNLQQIFQK IIQERGPFRD LKEEDLQKEL QKESIKDESS AKSSETENVL EFATLDSKR NVNDTEVESM DSQAYKKELI EQIMIAQTEC SLALDMTSLL LSKFKENSIE TISPFLKSTV PPSSLQFSRS Q PPESKESD ATLAKCWKEK SLTSSCKFLF EAKERLTSVV ETEHEYYTEL VKVKEASWPL FNSQGSNHLS VQYSCLGGIS LG LGLIRMK PESKSFEVQS SLLYSQAALK ISILNKDRDE IGSSTWSWPS QNCNSVLLKD IYKLQEILFE MDIWNSLLQE AQS CGNQGV NFTGDEILVP ISDDHVVRIT LETSSKNTES GFTEDKKSNE DTSTNFVTIK QEKELLKCLC DTLNAIAHIL FLKH CRKSD RRSQQPELYM AIDANAPLIL RPLIFYYNLN QESLEFQRWL KQRDISFKFM PNYPWEKAKD FLELENSLSI NRLSI SWRI MVSNFEPAIF IQHTPTLHGT DKSVWRCKDQ YSSNQFSSLK NVCQYIEHHI NSLSRRSKKT E

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

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分子 #4: Mediator complex subunit 18

分子名称: Mediator complex subunit 18 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 24.056377 KDa
配列文字列: MQELYLLGVV PSRRFEAVVN SLSKTLDGPK TILEFWVVYR PKDVPPNLPR QPDSWLRLCS NIESHDETDT EWSKNTQWSM YLEGNSEPK REDKCGIRPV NRAKLTNGSV TEFVEKMGYE FSHEYIIQGL EYFFFDTTVR IYQTLIPSQQ RSIKPPFHPM N EEQPWILH ...文字列:
MQELYLLGVV PSRRFEAVVN SLSKTLDGPK TILEFWVVYR PKDVPPNLPR QPDSWLRLCS NIESHDETDT EWSKNTQWSM YLEGNSEPK REDKCGIRPV NRAKLTNGSV TEFVEKMGYE FSHEYIIQGL EYFFFDTTVR IYQTLIPSQQ RSIKPPFHPM N EEQPWILH VYTHVADASN QVAMAKAEAN LTKVKTLLSA FCDLKNVRL

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

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分子 #5: Mediator complex subunit 20

分子名称: Mediator complex subunit 20 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 22.374775 KDa
配列文字列: MPVHGVIYYS SPSMATFLSP AQDNLVRTYF AQHLKKWVVQ YKLYRNAVTP KTLEFLKQNI NPSMLACVDE ATMIDAEPEL EDIIVRTKL WNFRQSFTIE GSIYEVGSFK VAIANVLQKS VWKGILFHVT YDGTESVDLA RPIIQEFFLK CFLQNNKSVT P VYESFFNQ ...文字列:
MPVHGVIYYS SPSMATFLSP AQDNLVRTYF AQHLKKWVVQ YKLYRNAVTP KTLEFLKQNI NPSMLACVDE ATMIDAEPEL EDIIVRTKL WNFRQSFTIE GSIYEVGSFK VAIANVLQKS VWKGILFHVT YDGTESVDLA RPIIQEFFLK CFLQNNKSVT P VYESFFNQ PRHSLDSKLL LQLFKQRIDT VSQRT

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

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分子 #6: Mediator complex subunit 11

分子名称: Mediator complex subunit 11 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 13.1188 KDa
配列文字列:
MTNSDDDLFS EKSTSSDTQQ VQNILELEAK IPDILSSAGK CIEAIQLNNS LEDFRKYSKE FLETVEFIST GLRRQALELE KAEVPVVSL QPKKRYASTP LSNLIFDQSS KLIPKYC

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

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分子 #7: Mediator complex subunit 22

分子名称: Mediator complex subunit 22 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 15.396165 KDa
配列文字列:
MSSDSFQRQL VQRTNTLNSS IDNATLTILS RFQDILDIAI NEGKDKYTVA PEVYQIECHT VSMVRAVEQL LDVSRQIKSY WLTNSLSTS FPTVDYSEPD LEKVKRTLTK LQNHLLEVSL IEPEASETTE APTVSDT

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

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分子 #8: Mediator complex subunit 14

分子名称: Mediator complex subunit 14 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 105.382461 KDa
配列文字列: MEPPAIPHIT EGFYPLPEIV ETFSHHVLQE LVSLAEVLPS MSNVEKKKKI LDWLLRSRAF TMRLLVLARW VHLSPSVHRC IDVVAFLQG QKFCFQNLVH VLQDIRYQLS FARLRNSDLV TALDILSTGT SLRLANAPTS KLYMLSESPL STKQILQTLH A LNMLIRIR ...文字列:
MEPPAIPHIT EGFYPLPEIV ETFSHHVLQE LVSLAEVLPS MSNVEKKKKI LDWLLRSRAF TMRLLVLARW VHLSPSVHRC IDVVAFLQG QKFCFQNLVH VLQDIRYQLS FARLRNSDLV TALDILSTGT SLRLANAPTS KLYMLSESPL STKQILQTLH A LNMLIRIR LSLYEIIPTP FQHFTIANGR CTFTVPNEFS VSLTTNSQDP KSTGISFQWI VVDFQFHLPD FSSTPAKYRV FI ELHLNEE IAAAFVLQKP ILPLIYNILH KFCLYQRLNL LSQQTFQLSR ESWLGHLRGV YDEKPPRLRL YYWPQLNVKK EGK PGKIGH YIHIFVNTQP ISAFERTLSS KRSSCEYDHF LLLVEWHHDG IVEHVPLDDH MDAQHLLLLI TQKHAQLILE QIRK ELHPN IFSEHVGGGL KIHVFDNEII VKVNSVTGRL VLSSSASPLS PPRHLRAAEK NIALNTQPPA QILNRLYFFC IQTQL LEVA QCAELHAVQG YYSFPYLTFS KGKWRKDGDS LWVLAYNVES NSWSVRLLNA AGQTLYTQDV HTTKGTLSIE SFSRLS YLL EVQILLFNVQ TACQARGMPF EYLPIPPKAL IEDDFTTYVQ TGCLCIMMPS SNEDMLPVVF VRAHDGQLIF DSRIKGK LP YQSETETEKN CYIDWRTGRI TIRVQNFSSF EKTWIGLLKL VALSKTSAFN VDCITLKHVD FTYLDDEKFR ATIHDDNT F TLHFFNRHSP FHLISQFLQD TFSDGPSAIQ PLRVIMDRTR GVLVAQELGY VVLARSLRQY RIILSKNHGI QVLLNRHGC ILQDLSYLSA DSRYLEGTQT LTSQWEPCSW LNTVWEGDLG DDELNGQIEA APEMHLIKMN KTADLTAILK RILAISRKK (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

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分子 #9: Mediator complex subunit 4

分子名称: Mediator complex subunit 4 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 20.35801 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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分子 #10: Mediator complex subunit 7

分子名称: Mediator complex subunit 7 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 43.573094 KDa
配列文字列: MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA KLFKKPSCLT SGTYQMFGDT WRLDEAIPS LKEFGIPELY KDIKDGEDEI EVVEYDPKSN AIVGTSFTRV HDYDKNSPIE NEKILEDDQH TAMKEKDNES D KTMKDVEE ...文字列:
MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA KLFKKPSCLT SGTYQMFGDT WRLDEAIPS LKEFGIPELY KDIKDGEDEI EVVEYDPKSN AIVGTSFTRV HDYDKNSPIE NEKILEDDQH TAMKEKDNES D KTMKDVEE KTEPSLKKEE EDIQMKEPLD SQDTGAVSAS SVNEGFRADQ KSKDGETSDL IKIPRRAYEL RFLSRSLMLN FL ELLGIMA KAPEQFPSKV ENIRVLLLNL HHLINDYRPH QSRESLIMLL EKQLKHEESQ VELLRTHNRQ MTETLEKYKS LDF NMEKEG DVIQQLKSSI KKPLSGAEDE QKSRSMFSKN DEKLKKSLEL MEDVIKRDLS

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

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分子 #11: Mediator complex subunit 21

分子名称: Mediator complex subunit 21 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 15.821031 KDa
配列文字列:
MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD LAKDLVTKFM QIDTLINQLP GISTAPKHQ LEKIKKLQNS IEEKQLERKS LESENEDLKL QLAKRIETFG RLSCVLFQP

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

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分子 #12: Mediator complex subunit 31

分子名称: Mediator complex subunit 31 / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 16.913975 KDa
配列文字列:
METKWLLSKV PDDKSRFEIE LEFVQMLSNP WYLNFLAQHK YFEDEAFLQY LEYMEYWREP EYVKFIIYPT CLHMLTLLKN PQFRNDISR ADLSKQVNDE IYYEWLGKGL QQYGSADDAT LSQPQQEEDE KKVDVKKENE

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

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分子 #13: Mediator complex subunit 27

分子名称: Mediator complex subunit 27 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 31.132693 KDa
配列文字列: MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK VNSFYTHLKS SQNVFDYAEK SLQADSSNL LPTYLYNSED LSNDTENNET KSINGKSALD LKEPHHSELH DNDNFQNSDI NIESFKGDIE ASGSILTTHE N KSFTLKLA ...文字列:
MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK VNSFYTHLKS SQNVFDYAEK SLQADSSNL LPTYLYNSED LSNDTENNET KSINGKSALD LKEPHHSELH DNDNFQNSDI NIESFKGDIE ASGSILTTHE N KSFTLKLA NELEFIFFHD TRGKFSVYCS SSKDDAITFS INRNNNFLGN LWSLMPKILD YQHLYSKPCD FCKSLISPVY LE LPSVRRN ANSTVKPTSK DILALHAECV PAQSDL

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

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分子 #14: Mediator complex subunit 9

分子名称: Mediator complex subunit 9 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 13.810964 KDa
配列文字列:
MDPAHHIKDT SAELLSQQDF SILQSRLLEF LASQEARETV TASKELTLLR QGIRQLKEKV SKMEPEEMTV KEKKSIIEIL KARIALKKA FLKMALSESN DTVVKKEDVR ESPVDTFMEN AT

UniProtKB: Uncharacterized protein C24C9.04

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分子 #15: Mediator complex subunit 19

分子名称: Mediator complex subunit 19 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 11.847596 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #16: Mediator complex subunit 10

分子名称: Mediator complex subunit 10 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 11.251861 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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分子 #17: RNA polymerase II subunit Rpb1

分子名称: RNA polymerase II subunit Rpb1 / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA-directed RNA polymerase
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 194.372625 KDa
配列文字列: MSGIQFSPSS VPLRRVEEVQ FGILSPEEIR SMSVAKIEFP ETMDESGQRP RVGGLLDPRL GTIDRQFKCQ TCGETMADCP GHFGHIELA KPVFHIGFLS KIKKILECVC WNCGKLKIDS SNPKFNDTQR YRDPKNRLNA VWNVCKTKMV CDTGLSAGSD N FDLSNPSA ...文字列:
MSGIQFSPSS VPLRRVEEVQ FGILSPEEIR SMSVAKIEFP ETMDESGQRP RVGGLLDPRL GTIDRQFKCQ TCGETMADCP GHFGHIELA KPVFHIGFLS KIKKILECVC WNCGKLKIDS SNPKFNDTQR YRDPKNRLNA VWNVCKTKMV CDTGLSAGSD N FDLSNPSA NMGHGGCGAA QPTIRKDGLR LWGSWKRGKD ESDLPEKRLL SPLEVHTIFT HISSEDLAHL GLNEQYARPD WM IITVLPV PPPSVRPSIS VDGTSRGEDD LTHKLSDIIK ANANVRRCEQ EGAPAHIVSE YEQLLQFHVA TYMDNEIAGQ PQA LQKSGR PLKSIRARLK GKEGRLRGNL MGKRVDFSAR TVITGDPNLS LDELGVPRSI AKTLTYPETV TPYNIYQLQE LVRN GPDEH PGAKYIIRDT GERIDLRYHK RAGDIPLRYG WRVERHIRDG DVVIFNRQPS LHKMSMMGHR IRVMPYSTFR LNLSV TSPY NADFDGDEMN MHVPQSEETR AEIQEITMVP KQIVSPQSNK PVMGIVQDTL AGVRKFSLRD NFLTRNAVMN IMLWVP DWD GILPPPVILK PKVLWTGKQI LSLIIPKGIN LIRDDDKQSL SNPTDSGMLI ENGEIIYGVV DKKTVGASQG GLVHTIW KE KGPEICKGFF NGIQRVVNYW LLHNGFSIGI GDTIADADTM KEVTRTVKEA RRQVAECIQD AQHNRLKPEP GMTLRESF E AKVSRILNQA RDNAGRSAEH SLKDSNNVKQ MVAAGSKGSF INISQMSACV GQQIVEGKRI PFGFKYRTLP HFPKDDDSP ESRGFIENSY LRGLTPQEFF FHAMAGREGL IDTAVKTAET GYIQRRLVKA MEDVMVRYDG TVRNAMGDII QFAYGEDGLD ATLVEYQVF DSLRLSTKQF EKKYRIDLME DRSLSLYMEN SIENDSSVQD LLDEEYTQLV ADRELLCKFI FPKGDARWPL P VNVQRIIQ NALQIFHLEA KKPTDLLPSD IINGLNELIA KLTIFRGSDR ITRDVQNNAT LLFQILLRSK FAVKRVIMEY RL NKVAFEW IMGEVEARFQ QAVVSPGEMV GTLAAQSIGE PATQMTLNTF HYAGVSSKNV TLGVPRLKEI LNVAKNIKTP SLT IYLMPW IAANMDLAKN VQTQIEHTTL STVTSATEIH YDPDPQDTVI EEDKDFVEAF FAIPDEEVEE NLYKQSPWLL RLEL DRAKM LDKKLSMSDV AGKIAESFER DLFTIWSEDN ADKLIIRCRI IRDDDRKAED DDNMIEEDVF LKTIEGHMLE SISLR GVPN ITRVYMMEHK IVRQIEDGTF ERADEWVLET DGINLTEAMT VEGVDATRTY SNSFVEILQI LGIEATRSAL LKELRN VIE FDGSYVNYRH LALLCDVMTS RGHLMAITRH GINRAETGAL MRCSFEETVE ILMDAAASGE KDDCKGISEN IMLGQLA PM GTGAFDIYLD QDMLMNYSLG TAVPTLAGSG MGTSQLPEGA GTPYERSPMV DSGFVGSPDA AAFSPLVQGG SEGREGFG D YGLLGAASPY KGVQSPGYTS PFSSAMSPGY GLTSPSYSPS SPGYSTSPAY MPSSPSYSPT SPSYSPTSPS YSPTSPSYS PTSPSYSATS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPS

UniProtKB: DNA-directed RNA polymerase II subunit rpb1

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分子 #18: RNA polymerase II subunit Rpb2

分子名称: RNA polymerase II subunit Rpb2 / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA-directed RNA polymerase
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 138.027188 KDa
配列文字列: MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTM TEADGSTTTM FPQEARLRNL TYSSPLYVDM RKKVMVAADS NVPIGEEEWL VEEEDEEPSK VFIGKIPIML R STFCILNG ...文字列:
MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTM TEADGSTTTM FPQEARLRNL TYSSPLYVDM RKKVMVAADS NVPIGEEEWL VEEEDEEPSK VFIGKIPIML R STFCILNG VSDSELYDLN ECPYDQGGYF IINGSEKVII AQERSAANIV QVFKKAAPSP IAYVAEIRSA LERGSRLISS MQ IKLMARN TENSGQTIRA TLPYIRSDIP IVIVFRALGV VPDRDILEHI CYDPNDFQML EMMKPCIEEA FVIQDKDIAL DYI GKRGST TGVTREKRLR YAHDILQKEL LPHITTMEGF ETRKAFFLGY MIHRMLLCAL ERREPDDRDH FGKKRLDLAG PLLA SLFRM LFRKMTRDVY KYMQKCVETN REFNLTLAVK SNIITNGLRY SLATGNWGDQ KRSMVNRVGV SQVLNRYTFA STLSH LRRT NTPIGRDGKL AKPRQLHNTH WGMVCPAETP EGQACGLVKN LSLMSYVSVG SPSAPIIEFL EEWGLETLED YNPSAS PNA TKVFVNGVWL GVHRDPAHLT ETLRSLRRRL DISAEVSIVR DIREKELRLF TDAGRICRPL FIVDNNPNSE RRGELCI RK EHIQQLIEDK DRYDIDPEQR FGWTALVSSG LIEYLDAEEE ETVMIAMSPE DLEASRQMQA GYEVKEELDP AQRVKPAP N PHVHAWTHCE IHPAMILGIL ASIIPFPDHN QSPRNTYQSA MGKQAMGVYL TNYQVRMDTM ANILYYPQKP LATTRSMEY LKFRELPAGQ NAIVAILCYS GYNQEDSIIM NQASIDRGLF RSIFYRTYTD QEKKIGMTVM EEFERPVRST TLRMKHGTYD KLEDDGLIA PGTRVSGEDI IIGKTAPIPL DHEELGQRTQ LHAKRDVSTP LRSTESGIVD QVMVTTNQEG LKFVKVRMRS T RIPQIGDK FASRHGQKGT IGMTYRHEDM PFSAQGIVPD IIINPHAIPS RMTVAHLVEC QLSKVSALSG FEGDATPFTD VT VEAVSKL LRSHGFQSRG FEVMYHGHTG RKLVAQVFLG PTYYQRLKHL VDDKIHARAR GPVQILTRQP VEGRSRDGGL RFG EMERDC QISHGCSSVL RERLFDCSDA YRVIVCDICG LIAIASYKKD SYECRSCQNR TRFSQVYLPY AAKLLFQELM SMNI APRLF TKNHK

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

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分子 #19: RNA polymerase II subunit Rpb3

分子名称: RNA polymerase II subunit Rpb3 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 33.748816 KDa
配列文字列: MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRN CDCDQYCPKC SVELFLNAKC TGEGTMEIYA RDLVVSSNSS LGHPILADPK SRGPLICKLR KEQEISLRCI A KKGIAKEH ...文字列:
MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRN CDCDQYCPKC SVELFLNAKC TGEGTMEIYA RDLVVSSNSS LGHPILADPK SRGPLICKLR KEQEISLRCI A KKGIAKEH AKWSPTSAVA FEYDPWNKLQ HTDYWFENDA DAEWPKSKNA DWEEPPREGE PFNFQEEPRR FYMDVESVGS IP PNEIMVQ GLRILQEKLA VLVRDLDEEQ PTQLSANELN MEENAEMNWS PYQNGEENTW

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

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分子 #20: RNA polymerase II subunit Rpb4

分子名称: RNA polymerase II subunit Rpb4 / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 15.37949 KDa
配列文字列:
MPRAIFEEDA AQLKLGPEFE NEDMLTVSEA KILIETVLAQ RARETNGEIP MTDVMKKTVA YFNVFARFKT AEATYACERI LGNRFHKFE RAQLGTLCCE DAEEARTLIP SLANKIDDQN LQGILDELST LRKFQD

UniProtKB: DNA-directed RNA polymerase II subunit rpb4

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分子 #21: RNA polymerase II subunit Rpb5

分子名称: RNA polymerase II subunit Rpb5 / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 23.954504 KDa
配列文字列: MSAEEKNIVR VFRAWKTAHQ LVHDRGYGVS QAELDLTLDQ FKAMHCGMGR NLDRTTLSFY AKPSNDSNKG TIYIEFAKEP SVGIKEMRT FVHTLGDHNH KTGILIYANS MTPSAAKIIA TVTGQFTIET FQESDLIVNI THHELVPKHI LLSPDEKKEL L DRYKLRET ...文字列:
MSAEEKNIVR VFRAWKTAHQ LVHDRGYGVS QAELDLTLDQ FKAMHCGMGR NLDRTTLSFY AKPSNDSNKG TIYIEFAKEP SVGIKEMRT FVHTLGDHNH KTGILIYANS MTPSAAKIIA TVTGQFTIET FQESDLIVNI THHELVPKHI LLSPDEKKEL L DRYKLRET QLPRIQLADP VARYLGLKRG EVVKIVRRSE TSGRYNSYRI CA

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

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分子 #22: RNA polymerase II subunit Rpb6

分子名称: RNA polymerase II subunit Rpb6 / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 15.742497 KDa
配列文字列:
MSDYEEDEAF GMDGAVMEEE VDELEMIDEN GQSQQGVSHP GEPSTTVITE DVASSKTAQS GKAVAKEDRT TTPYMTKYER ARILGTRAL QISMNAPVLV DLEGETDPLQ IAMKELAQKK IPLLVRRYLP DGSYEDWSVA ELI

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

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分子 #23: RNA polymerase II subunit Rpb7

分子名称: RNA polymerase II subunit Rpb7 / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 19.121982 KDa
配列文字列:
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVD AIVTTVNKMG FFANIGPLNV FVSSHLVPPD MKFDPTANPP NYSGEDQVIE KGSNVRLKIV GTRTDATEIF A IATMKEDY LGVL

UniProtKB: DNA-directed RNA polymerase II subunit rpb7

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分子 #24: RNA polymerase II subunit Rpb8

分子名称: RNA polymerase II subunit Rpb8 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 14.317318 KDa
配列文字列:
MSESVLLDEI FTVTSVDKQK YQRVSRITAV SGQNDMNLTL DINSQIYPLE KDATFSLQIT SNLNSPDLKE AADYIMYGKV YRVEEAKDE KVSVYVSFGG LLMAIEGSHR KLYRLSLDHV YLLLRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

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分子 #25: RNA polymerase II subunit Rpb9

分子名称: RNA polymerase II subunit Rpb9 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 13.192771 KDa
配列文字列:
MSNFQYCIEC NNMLYPREDK VDRVLRLACR NCDYSEIAAT SKVYRHELQS SNVENTTVSH DASTDPTLPR SDKECPRCHQ HEAVFYQTH SRRGDTMMTL IYVCVHCGFA FEEQ

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

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分子 #26: RNA polymerase II subunit Rpb10

分子名称: RNA polymerase II subunit Rpb10 / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 8.286801 KDa
配列文字列:
MIIPIRCFSC GKVIGDKWDT YLTLLQEDNT EGEALDKLGL QRYCCRRMIL THVDLIEKLL CYNPLSKQKN L

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

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分子 #27: RNA polymerase II subunit Rpb11

分子名称: RNA polymerase II subunit Rpb11 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 14.143276 KDa
配列文字列:
MNQPERYELI ELMGLPKVTY ELDSKSPNAA VVTLEKEDHT LANMLANQLL SDERVLFAGY KVPHPLNHNF ILRVQTVEDC SPKQVIVDA AKSLITHLEE IKVNFMREWE LKMISVEGVE MEFS

UniProtKB: DNA-directed RNA polymerase II subunit RPB11

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分子 #28: RNA polymerase II subunit Rpb12

分子名称: RNA polymerase II subunit Rpb12 / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Schizosaccharomyces pombe (分裂酵母)
分子量理論値: 7.216495 KDa
配列文字列:
MNHPTSTGGT AFNPPRPATM IYLCADCGAR NTIQAKEVIR CRECGHRVMY KMRTKRMVQF EAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

濃度1 mg/mL
緩衝液pH: 7.4
詳細: 20 mM HEPES, pH 7.4, 200 mM potassium acetate, 5 mM b-Mercaptoethanol, 0.01% NP-40
グリッドモデル: C-flat-2/2 4C / 材質: COPPER / メッシュ: 400
凍結凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: HOMEMADE PLUNGER

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
撮影フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k)
検出モード: COUNTING / 平均露光時間: 7.0 sec. / 平均電子線量: 9.6 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系C2レンズ絞り径: 100.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 4.0 µm / 最小 デフォーカス（公称値）: 1.0 µm / 倍率（公称値）: 22500
試料ステージ試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
ホルダー冷却材: NITROGEN
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

初期モデルモデルのタイプ: NONE
最終 再構成想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 7.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 1.4) / 使用した粒子像数: 3862
初期 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 1.4)
最終 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 1.4)
FSC曲線 (解像度の算出)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る