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TitleMediator structure and rearrangements required for holoenzyme formation.
Journal, issue, pagesNature, Vol. 544, Issue 7649, Page 196-201, Year 2017
Publish dateApr 13, 2017
AuthorsKuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias /
PubMed AbstractThe conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is ...The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.
External linksNature / PubMed:28241144 / PubMed Central
MethodsEM (single particle)
Resolution4.4 - 7.8 Å
Structure data

8479

5u0p

EMDB-8479: Cryo-EM map of the transcriptional Mediator
PDB-5u0p: Cryo-EM structure of the transcriptional Mediator
Method: EM (single particle) / Resolution: 4.4 Å

8480

5u0s

EMDB-8480: Cryo-EM map of the Mediator-RNAPII complex
PDB-5u0s: Cryo-EM structure of the Mediator-RNAPII complex
Method: EM (single particle) / Resolution: 7.8 Å

Source
  • schizosaccharomyces pombe (fission yeast)
  • Fission yeast (fission yeast)
KeywordsTRANSCRIPTION / transcriptional / Mediator / Head / Middle / Tail / Srb / RNA polymerase II / Med / TRANSCRIPTION/TRANSFERASE / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex

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