EMDB-8480: Cryo-EM map of the Mediator-RNAPII complex

Basic information

• Entry: Database: EMDB / ID: EMD-8480
• Title: Cryo-EM map of the Mediator-RNAPII complex
• Map data: Mediator-RNAPII complex

Sample

• Complex: complex of Mediator and RNAPII
  • Protein or peptide: x 28 types

• Keywords: transcriptional / transcription / Mediator / Srb / RNA polymerase II / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex

Function / homology

Function:

RNA Polymerase I Transcription Initiation / RNA polymerase II, holoenzyme / RNA polymerase II transcribes snRNA genes / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / mRNA Capping / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Estrogen-dependent gene expression / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway / RNA Polymerase I Promoter Escape / mRNA Splicing - Major Pathway / Transcriptional regulation by small RNAs / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / regulation of septum digestion after cytokinesis / siRNA-mediated pericentric heterochromatin formation / core mediator complex / DNA-templated transcription elongation / intracellular phosphate ion homeostasis / mediator complex / chromatin-protein adaptor activity / termination of RNA polymerase II transcription / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / nuclear-transcribed mRNA catabolic process / transcription by RNA polymerase III / positive regulation of translational initiation / 7-methylguanosine mRNA capping / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / pericentric heterochromatin / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / translation initiation factor binding / transcription-coupled nucleotide-excision repair / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / P-body / euchromatin / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / single-stranded DNA binding / nucleic acid binding / transcription by RNA polymerase II / transcription coactivator activity / single-stranded RNA binding / protein dimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / nucleotide binding / regulation of transcription by RNA polymerase II / chromatin / nucleolus / mitochondrion / DNA binding / RNA binding / zinc ion binding / metal ion binding / nucleus / cytoplasm / cytosol

Domain/homology:

Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / Rpb4/RPC9 superfamily / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerase subunit Rpb7-like / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / HRDC-like superfamily / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / : / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase subunit CX / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo11 / RNA polymerases L / 13 to 16 Kd subunits signature. / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site

Component:

DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Uncharacterized protein C24C9.04 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / DNA-directed RNA polymerase II subunit rpb7 / Mediator of RNA polymerase II transcription subunit 7 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit rpb4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 8 / DNA-directed RNA polymerase II subunit rpb1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase II subunit RPB11 / Mediator of RNA polymerase II transcription subunit 17 / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 27 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 31

• Biological species: Schizosaccharomyces pombe (fission yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 7.8 Å
• Authors: Tsai K-L / Yu X

Funding support

United States, 2 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM67167	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM41628	United States

• Citation: Journal: Nature / Year: 2017; Title: Mediator structure and rearrangements required for holoenzyme formation.; Authors: Kuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias / ; Abstract: The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.

History

Deposition	Nov 26, 2016	-
Header (metadata) release	Mar 8, 2017	-
Map release	Mar 8, 2017	-
Update	Nov 20, 2024	-
Current status	Nov 20, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_8480.map.gz / Format: CCP4 / Size: 20.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Mediator-RNAPII complex
• Voxel size: X=Y=Z: 2.62 Å

Density

Contour Level	By EMDB: 0.08 / Movie #1: 0.08
Minimum - Maximum	-0.11419473 - 0.22855034
Average (Standard dev.)	-0.000103825936 (±0.014926006)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 176 176 176 Spacing 176 176 176
Cell	A=B=C: 461.12 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	2.62	2.62	2.62
M x/y/z	176	176	176
origin x/y/z	0.000	0.000	0.000
length x/y/z	461.120	461.120	461.120
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	176	176	176
D min/max/mean	-0.114	0.229	-0.000

Supplemental data

Sample components

Entire : complex of Mediator and RNAPII

• Entire: Name: complex of Mediator and RNAPII

Components

• Complex: complex of Mediator and RNAPII
  • Protein or peptide: Mediator complex subunit 6
  • Protein or peptide: Mediator complex subunit 8
  • Protein or peptide: Mediator complex subunit 17
  • Protein or peptide: Mediator complex subunit 18
  • Protein or peptide: Mediator complex subunit 20
  • Protein or peptide: Mediator complex subunit 11
  • Protein or peptide: Mediator complex subunit 22
  • Protein or peptide: Mediator complex subunit 14
  • Protein or peptide: Mediator complex subunit 4
  • Protein or peptide: Mediator complex subunit 7
  • Protein or peptide: Mediator complex subunit 21
  • Protein or peptide: Mediator complex subunit 31
  • Protein or peptide: Mediator complex subunit 27
  • Protein or peptide: Mediator complex subunit 9
  • Protein or peptide: Mediator complex subunit 19
  • Protein or peptide: Mediator complex subunit 10
  • Protein or peptide: RNA polymerase II subunit Rpb1
  • Protein or peptide: RNA polymerase II subunit Rpb2
  • Protein or peptide: RNA polymerase II subunit Rpb3
  • Protein or peptide: RNA polymerase II subunit Rpb4
  • Protein or peptide: RNA polymerase II subunit Rpb5
  • Protein or peptide: RNA polymerase II subunit Rpb6
  • Protein or peptide: RNA polymerase II subunit Rpb7
  • Protein or peptide: RNA polymerase II subunit Rpb8
  • Protein or peptide: RNA polymerase II subunit Rpb9
  • Protein or peptide: RNA polymerase II subunit Rpb10
  • Protein or peptide: RNA polymerase II subunit Rpb11
  • Protein or peptide: RNA polymerase II subunit Rpb12

Supramolecule #1: complex of Mediator and RNAPII

• Supramolecule: Name: complex of Mediator and RNAPII / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 1.5 MDa

Macromolecule #1: Mediator complex subunit 6

• Macromolecule: Name: Mediator complex subunit 6 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 24.928947 KDa

Sequence

String:

MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLW VIQKQNRLNE NEVKPLTVYF VCNENIYMAP NAYTLLATRM LNATYCFQKA LTKIEKFPQY NPQEGYTYPK L SNDNLEVD HSNTNEPADE NKNQSIENAD YSFSPEDFSV VRAFMQSLHS SKEAPDVK

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

Macromolecule #2: Mediator complex subunit 8

• Macromolecule: Name: Mediator complex subunit 8 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 23.360068 KDa

Sequence

String:

MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL SNNLAAHSHT LQTTSIYPSL EFPVKEQEP LLTTLLRTKA LPEVEEWEAN TLQEYEASIS SQPKKKEAND AYQKDQLWDQ ARIIFMEERE NYSWFDFVTR R QESEGEFV SQRQLEIDRA TEEQNANQML TDILSFMKSG KR

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

Macromolecule #3: Mediator complex subunit 17

• Macromolecule: Name: Mediator complex subunit 17 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 62.55141 KDa

Sequence

String:

MAEEANKDAD ISSLSLSLDP EIIGGQNNFL ENNLQQIFQK IIQERGPFRD LKEEDLQKEL QKESIKDESS AKSSETENVL EFATLDSKR NVNDTEVESM DSQAYKKELI EQIMIAQTEC SLALDMTSLL LSKFKENSIE TISPFLKSTV PPSSLQFSRS Q PPESKESD ATLAKCWKEK SLTSSCKFLF EAKERLTSVV ETEHEYYTEL VKVKEASWPL FNSQGSNHLS VQYSCLGGIS LG LGLIRMK PESKSFEVQS SLLYSQAALK ISILNKDRDE IGSSTWSWPS QNCNSVLLKD IYKLQEILFE MDIWNSLLQE AQS CGNQGV NFTGDEILVP ISDDHVVRIT LETSSKNTES GFTEDKKSNE DTSTNFVTIK QEKELLKCLC DTLNAIAHIL FLKH CRKSD RRSQQPELYM AIDANAPLIL RPLIFYYNLN QESLEFQRWL KQRDISFKFM PNYPWEKAKD FLELENSLSI NRLSI SWRI MVSNFEPAIF IQHTPTLHGT DKSVWRCKDQ YSSNQFSSLK NVCQYIEHHI NSLSRRSKKT E

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

Macromolecule #4: Mediator complex subunit 18

• Macromolecule: Name: Mediator complex subunit 18 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 24.056377 KDa

Sequence

String:

MQELYLLGVV PSRRFEAVVN SLSKTLDGPK TILEFWVVYR PKDVPPNLPR QPDSWLRLCS NIESHDETDT EWSKNTQWSM YLEGNSEPK REDKCGIRPV NRAKLTNGSV TEFVEKMGYE FSHEYIIQGL EYFFFDTTVR IYQTLIPSQQ RSIKPPFHPM N EEQPWILH VYTHVADASN QVAMAKAEAN LTKVKTLLSA FCDLKNVRL

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

Macromolecule #5: Mediator complex subunit 20

• Macromolecule: Name: Mediator complex subunit 20 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 22.374775 KDa

Sequence

String:

MPVHGVIYYS SPSMATFLSP AQDNLVRTYF AQHLKKWVVQ YKLYRNAVTP KTLEFLKQNI NPSMLACVDE ATMIDAEPEL EDIIVRTKL WNFRQSFTIE GSIYEVGSFK VAIANVLQKS VWKGILFHVT YDGTESVDLA RPIIQEFFLK CFLQNNKSVT P VYESFFNQ PRHSLDSKLL LQLFKQRIDT VSQRT

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

Macromolecule #6: Mediator complex subunit 11

• Macromolecule: Name: Mediator complex subunit 11 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 13.1188 KDa

Sequence

String:

MTNSDDDLFS EKSTSSDTQQ VQNILELEAK IPDILSSAGK CIEAIQLNNS LEDFRKYSKE FLETVEFIST GLRRQALELE KAEVPVVSL QPKKRYASTP LSNLIFDQSS KLIPKYC

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

Macromolecule #7: Mediator complex subunit 22

• Macromolecule: Name: Mediator complex subunit 22 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 15.396165 KDa

Sequence

String:

MSSDSFQRQL VQRTNTLNSS IDNATLTILS RFQDILDIAI NEGKDKYTVA PEVYQIECHT VSMVRAVEQL LDVSRQIKSY WLTNSLSTS FPTVDYSEPD LEKVKRTLTK LQNHLLEVSL IEPEASETTE APTVSDT

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

Macromolecule #8: Mediator complex subunit 14

• Macromolecule: Name: Mediator complex subunit 14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 105.382461 KDa

Sequence

String:

MEPPAIPHIT EGFYPLPEIV ETFSHHVLQE LVSLAEVLPS MSNVEKKKKI LDWLLRSRAF TMRLLVLARW VHLSPSVHRC IDVVAFLQG QKFCFQNLVH VLQDIRYQLS FARLRNSDLV TALDILSTGT SLRLANAPTS KLYMLSESPL STKQILQTLH A LNMLIRIR LSLYEIIPTP FQHFTIANGR CTFTVPNEFS VSLTTNSQDP KSTGISFQWI VVDFQFHLPD FSSTPAKYRV FI ELHLNEE IAAAFVLQKP ILPLIYNILH KFCLYQRLNL LSQQTFQLSR ESWLGHLRGV YDEKPPRLRL YYWPQLNVKK EGK PGKIGH YIHIFVNTQP ISAFERTLSS KRSSCEYDHF LLLVEWHHDG IVEHVPLDDH MDAQHLLLLI TQKHAQLILE QIRK ELHPN IFSEHVGGGL KIHVFDNEII VKVNSVTGRL VLSSSASPLS PPRHLRAAEK NIALNTQPPA QILNRLYFFC IQTQL LEVA QCAELHAVQG YYSFPYLTFS KGKWRKDGDS LWVLAYNVES NSWSVRLLNA AGQTLYTQDV HTTKGTLSIE SFSRLS YLL EVQILLFNVQ TACQARGMPF EYLPIPPKAL IEDDFTTYVQ TGCLCIMMPS SNEDMLPVVF VRAHDGQLIF DSRIKGK LP YQSETETEKN CYIDWRTGRI TIRVQNFSSF EKTWIGLLKL VALSKTSAFN VDCITLKHVD FTYLDDEKFR ATIHDDNT F TLHFFNRHSP FHLISQFLQD TFSDGPSAIQ PLRVIMDRTR GVLVAQELGY VVLARSLRQY RIILSKNHGI QVLLNRHGC ILQDLSYLSA DSRYLEGTQT LTSQWEPCSW LNTVWEGDLG DDELNGQIEA APEMHLIKMN KTADLTAILK RILAISRKK (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

Macromolecule #9: Mediator complex subunit 4

• Macromolecule: Name: Mediator complex subunit 4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 20.35801 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #10: Mediator complex subunit 7

• Macromolecule: Name: Mediator complex subunit 7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 43.573094 KDa

Sequence

String:

MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA KLFKKPSCLT SGTYQMFGDT WRLDEAIPS LKEFGIPELY KDIKDGEDEI EVVEYDPKSN AIVGTSFTRV HDYDKNSPIE NEKILEDDQH TAMKEKDNES D KTMKDVEE KTEPSLKKEE EDIQMKEPLD SQDTGAVSAS SVNEGFRADQ KSKDGETSDL IKIPRRAYEL RFLSRSLMLN FL ELLGIMA KAPEQFPSKV ENIRVLLLNL HHLINDYRPH QSRESLIMLL EKQLKHEESQ VELLRTHNRQ MTETLEKYKS LDF NMEKEG DVIQQLKSSI KKPLSGAEDE QKSRSMFSKN DEKLKKSLEL MEDVIKRDLS

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

Macromolecule #11: Mediator complex subunit 21

• Macromolecule: Name: Mediator complex subunit 21 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 15.821031 KDa

Sequence

String:

MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD LAKDLVTKFM QIDTLINQLP GISTAPKHQ LEKIKKLQNS IEEKQLERKS LESENEDLKL QLAKRIETFG RLSCVLFQP

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

Macromolecule #12: Mediator complex subunit 31

• Macromolecule: Name: Mediator complex subunit 31 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 16.913975 KDa

Sequence

String:

METKWLLSKV PDDKSRFEIE LEFVQMLSNP WYLNFLAQHK YFEDEAFLQY LEYMEYWREP EYVKFIIYPT CLHMLTLLKN PQFRNDISR ADLSKQVNDE IYYEWLGKGL QQYGSADDAT LSQPQQEEDE KKVDVKKENE

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

Macromolecule #13: Mediator complex subunit 27

• Macromolecule: Name: Mediator complex subunit 27 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 31.132693 KDa

Sequence

String:

MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK VNSFYTHLKS SQNVFDYAEK SLQADSSNL LPTYLYNSED LSNDTENNET KSINGKSALD LKEPHHSELH DNDNFQNSDI NIESFKGDIE ASGSILTTHE N KSFTLKLA NELEFIFFHD TRGKFSVYCS SSKDDAITFS INRNNNFLGN LWSLMPKILD YQHLYSKPCD FCKSLISPVY LE LPSVRRN ANSTVKPTSK DILALHAECV PAQSDL

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

Macromolecule #14: Mediator complex subunit 9

• Macromolecule: Name: Mediator complex subunit 9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 13.810964 KDa

Sequence

String:

MDPAHHIKDT SAELLSQQDF SILQSRLLEF LASQEARETV TASKELTLLR QGIRQLKEKV SKMEPEEMTV KEKKSIIEIL KARIALKKA FLKMALSESN DTVVKKEDVR ESPVDTFMEN AT

UniProtKB: Uncharacterized protein C24C9.04

Macromolecule #15: Mediator complex subunit 19

• Macromolecule: Name: Mediator complex subunit 19 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 11.847596 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #16: Mediator complex subunit 10

• Macromolecule: Name: Mediator complex subunit 10 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 11.251861 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #17: RNA polymerase II subunit Rpb1

• Macromolecule: Name: RNA polymerase II subunit Rpb1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 194.372625 KDa

Sequence

String:

MSGIQFSPSS VPLRRVEEVQ FGILSPEEIR SMSVAKIEFP ETMDESGQRP RVGGLLDPRL GTIDRQFKCQ TCGETMADCP GHFGHIELA KPVFHIGFLS KIKKILECVC WNCGKLKIDS SNPKFNDTQR YRDPKNRLNA VWNVCKTKMV CDTGLSAGSD N FDLSNPSA NMGHGGCGAA QPTIRKDGLR LWGSWKRGKD ESDLPEKRLL SPLEVHTIFT HISSEDLAHL GLNEQYARPD WM IITVLPV PPPSVRPSIS VDGTSRGEDD LTHKLSDIIK ANANVRRCEQ EGAPAHIVSE YEQLLQFHVA TYMDNEIAGQ PQA LQKSGR PLKSIRARLK GKEGRLRGNL MGKRVDFSAR TVITGDPNLS LDELGVPRSI AKTLTYPETV TPYNIYQLQE LVRN GPDEH PGAKYIIRDT GERIDLRYHK RAGDIPLRYG WRVERHIRDG DVVIFNRQPS LHKMSMMGHR IRVMPYSTFR LNLSV TSPY NADFDGDEMN MHVPQSEETR AEIQEITMVP KQIVSPQSNK PVMGIVQDTL AGVRKFSLRD NFLTRNAVMN IMLWVP DWD GILPPPVILK PKVLWTGKQI LSLIIPKGIN LIRDDDKQSL SNPTDSGMLI ENGEIIYGVV DKKTVGASQG GLVHTIW KE KGPEICKGFF NGIQRVVNYW LLHNGFSIGI GDTIADADTM KEVTRTVKEA RRQVAECIQD AQHNRLKPEP GMTLRESF E AKVSRILNQA RDNAGRSAEH SLKDSNNVKQ MVAAGSKGSF INISQMSACV GQQIVEGKRI PFGFKYRTLP HFPKDDDSP ESRGFIENSY LRGLTPQEFF FHAMAGREGL IDTAVKTAET GYIQRRLVKA MEDVMVRYDG TVRNAMGDII QFAYGEDGLD ATLVEYQVF DSLRLSTKQF EKKYRIDLME DRSLSLYMEN SIENDSSVQD LLDEEYTQLV ADRELLCKFI FPKGDARWPL P VNVQRIIQ NALQIFHLEA KKPTDLLPSD IINGLNELIA KLTIFRGSDR ITRDVQNNAT LLFQILLRSK FAVKRVIMEY RL NKVAFEW IMGEVEARFQ QAVVSPGEMV GTLAAQSIGE PATQMTLNTF HYAGVSSKNV TLGVPRLKEI LNVAKNIKTP SLT IYLMPW IAANMDLAKN VQTQIEHTTL STVTSATEIH YDPDPQDTVI EEDKDFVEAF FAIPDEEVEE NLYKQSPWLL RLEL DRAKM LDKKLSMSDV AGKIAESFER DLFTIWSEDN ADKLIIRCRI IRDDDRKAED DDNMIEEDVF LKTIEGHMLE SISLR GVPN ITRVYMMEHK IVRQIEDGTF ERADEWVLET DGINLTEAMT VEGVDATRTY SNSFVEILQI LGIEATRSAL LKELRN VIE FDGSYVNYRH LALLCDVMTS RGHLMAITRH GINRAETGAL MRCSFEETVE ILMDAAASGE KDDCKGISEN IMLGQLA PM GTGAFDIYLD QDMLMNYSLG TAVPTLAGSG MGTSQLPEGA GTPYERSPMV DSGFVGSPDA AAFSPLVQGG SEGREGFG D YGLLGAASPY KGVQSPGYTS PFSSAMSPGY GLTSPSYSPS SPGYSTSPAY MPSSPSYSPT SPSYSPTSPS YSPTSPSYS PTSPSYSATS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPS

UniProtKB: DNA-directed RNA polymerase II subunit rpb1

Macromolecule #18: RNA polymerase II subunit Rpb2

• Macromolecule: Name: RNA polymerase II subunit Rpb2 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 138.027188 KDa

Sequence

String:

MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTM TEADGSTTTM FPQEARLRNL TYSSPLYVDM RKKVMVAADS NVPIGEEEWL VEEEDEEPSK VFIGKIPIML R STFCILNG VSDSELYDLN ECPYDQGGYF IINGSEKVII AQERSAANIV QVFKKAAPSP IAYVAEIRSA LERGSRLISS MQ IKLMARN TENSGQTIRA TLPYIRSDIP IVIVFRALGV VPDRDILEHI CYDPNDFQML EMMKPCIEEA FVIQDKDIAL DYI GKRGST TGVTREKRLR YAHDILQKEL LPHITTMEGF ETRKAFFLGY MIHRMLLCAL ERREPDDRDH FGKKRLDLAG PLLA SLFRM LFRKMTRDVY KYMQKCVETN REFNLTLAVK SNIITNGLRY SLATGNWGDQ KRSMVNRVGV SQVLNRYTFA STLSH LRRT NTPIGRDGKL AKPRQLHNTH WGMVCPAETP EGQACGLVKN LSLMSYVSVG SPSAPIIEFL EEWGLETLED YNPSAS PNA TKVFVNGVWL GVHRDPAHLT ETLRSLRRRL DISAEVSIVR DIREKELRLF TDAGRICRPL FIVDNNPNSE RRGELCI RK EHIQQLIEDK DRYDIDPEQR FGWTALVSSG LIEYLDAEEE ETVMIAMSPE DLEASRQMQA GYEVKEELDP AQRVKPAP N PHVHAWTHCE IHPAMILGIL ASIIPFPDHN QSPRNTYQSA MGKQAMGVYL TNYQVRMDTM ANILYYPQKP LATTRSMEY LKFRELPAGQ NAIVAILCYS GYNQEDSIIM NQASIDRGLF RSIFYRTYTD QEKKIGMTVM EEFERPVRST TLRMKHGTYD KLEDDGLIA PGTRVSGEDI IIGKTAPIPL DHEELGQRTQ LHAKRDVSTP LRSTESGIVD QVMVTTNQEG LKFVKVRMRS T RIPQIGDK FASRHGQKGT IGMTYRHEDM PFSAQGIVPD IIINPHAIPS RMTVAHLVEC QLSKVSALSG FEGDATPFTD VT VEAVSKL LRSHGFQSRG FEVMYHGHTG RKLVAQVFLG PTYYQRLKHL VDDKIHARAR GPVQILTRQP VEGRSRDGGL RFG EMERDC QISHGCSSVL RERLFDCSDA YRVIVCDICG LIAIASYKKD SYECRSCQNR TRFSQVYLPY AAKLLFQELM SMNI APRLF TKNHK

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

Macromolecule #19: RNA polymerase II subunit Rpb3

• Macromolecule: Name: RNA polymerase II subunit Rpb3 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 33.748816 KDa

Sequence

String:

MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRN CDCDQYCPKC SVELFLNAKC TGEGTMEIYA RDLVVSSNSS LGHPILADPK SRGPLICKLR KEQEISLRCI A KKGIAKEH AKWSPTSAVA FEYDPWNKLQ HTDYWFENDA DAEWPKSKNA DWEEPPREGE PFNFQEEPRR FYMDVESVGS IP PNEIMVQ GLRILQEKLA VLVRDLDEEQ PTQLSANELN MEENAEMNWS PYQNGEENTW

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #20: RNA polymerase II subunit Rpb4

• Macromolecule: Name: RNA polymerase II subunit Rpb4 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 15.37949 KDa

Sequence

String:

MPRAIFEEDA AQLKLGPEFE NEDMLTVSEA KILIETVLAQ RARETNGEIP MTDVMKKTVA YFNVFARFKT AEATYACERI LGNRFHKFE RAQLGTLCCE DAEEARTLIP SLANKIDDQN LQGILDELST LRKFQD

UniProtKB: DNA-directed RNA polymerase II subunit rpb4

Macromolecule #21: RNA polymerase II subunit Rpb5

• Macromolecule: Name: RNA polymerase II subunit Rpb5 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 23.954504 KDa

Sequence

String:

MSAEEKNIVR VFRAWKTAHQ LVHDRGYGVS QAELDLTLDQ FKAMHCGMGR NLDRTTLSFY AKPSNDSNKG TIYIEFAKEP SVGIKEMRT FVHTLGDHNH KTGILIYANS MTPSAAKIIA TVTGQFTIET FQESDLIVNI THHELVPKHI LLSPDEKKEL L DRYKLRET QLPRIQLADP VARYLGLKRG EVVKIVRRSE TSGRYNSYRI CA

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #22: RNA polymerase II subunit Rpb6

• Macromolecule: Name: RNA polymerase II subunit Rpb6 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 15.742497 KDa

Sequence

String:

MSDYEEDEAF GMDGAVMEEE VDELEMIDEN GQSQQGVSHP GEPSTTVITE DVASSKTAQS GKAVAKEDRT TTPYMTKYER ARILGTRAL QISMNAPVLV DLEGETDPLQ IAMKELAQKK IPLLVRRYLP DGSYEDWSVA ELI

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #23: RNA polymerase II subunit Rpb7

• Macromolecule: Name: RNA polymerase II subunit Rpb7 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 19.121982 KDa

Sequence

String:

MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVD AIVTTVNKMG FFANIGPLNV FVSSHLVPPD MKFDPTANPP NYSGEDQVIE KGSNVRLKIV GTRTDATEIF A IATMKEDY LGVL

UniProtKB: DNA-directed RNA polymerase II subunit rpb7

Macromolecule #24: RNA polymerase II subunit Rpb8

• Macromolecule: Name: RNA polymerase II subunit Rpb8 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 14.317318 KDa

Sequence

String:

MSESVLLDEI FTVTSVDKQK YQRVSRITAV SGQNDMNLTL DINSQIYPLE KDATFSLQIT SNLNSPDLKE AADYIMYGKV YRVEEAKDE KVSVYVSFGG LLMAIEGSHR KLYRLSLDHV YLLLRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #25: RNA polymerase II subunit Rpb9

• Macromolecule: Name: RNA polymerase II subunit Rpb9 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 13.192771 KDa

Sequence

String:

MSNFQYCIEC NNMLYPREDK VDRVLRLACR NCDYSEIAAT SKVYRHELQS SNVENTTVSH DASTDPTLPR SDKECPRCHQ HEAVFYQTH SRRGDTMMTL IYVCVHCGFA FEEQ

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #26: RNA polymerase II subunit Rpb10

• Macromolecule: Name: RNA polymerase II subunit Rpb10 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 8.286801 KDa

Sequence

String:

MIIPIRCFSC GKVIGDKWDT YLTLLQEDNT EGEALDKLGL QRYCCRRMIL THVDLIEKLL CYNPLSKQKN L

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #27: RNA polymerase II subunit Rpb11

• Macromolecule: Name: RNA polymerase II subunit Rpb11 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 14.143276 KDa

Sequence

String:

MNQPERYELI ELMGLPKVTY ELDSKSPNAA VVTLEKEDHT LANMLANQLL SDERVLFAGY KVPHPLNHNF ILRVQTVEDC SPKQVIVDA AKSLITHLEE IKVNFMREWE LKMISVEGVE MEFS

UniProtKB: DNA-directed RNA polymerase II subunit RPB11

Macromolecule #28: RNA polymerase II subunit Rpb12

• Macromolecule: Name: RNA polymerase II subunit Rpb12 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Schizosaccharomyces pombe (fission yeast)
• Molecular weight: Theoretical: 7.216495 KDa

Sequence

String:

MNHPTSTGGT AFNPPRPATM IYLCADCGAR NTIQAKEVIR CRECGHRVMY KMRTKRMVQF EAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 1 mg/mL
• Buffer: pH: 7.4 ; Details: 20 mM HEPES, pH 7.4, 200 mM potassium acetate, 5 mM b-Mercaptoethanol, 0.01% NP-40
• Grid: Model: C-flat-2/2 4C / Material: COPPER / Mesh: 400
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: HOMEMADE PLUNGER

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average exposure time: 7.0 sec. / Average electron dose: 9.6 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 22500
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: NONE
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 3862
• Initial angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)