5U0P

Cryo-EM structure of the transcriptional Mediator

5U0P の概要

• エントリーDOI: 10.2210/pdb5u0p/pdb
• 関連するPDBエントリー: 5U0S
• EMDBエントリー: 8479 8480
• 分子名称: Mediator complex subunit 14, Mediator complex subunit 7, Mediator complex subunit 21, ... (16 entities in total)
• 機能のキーワード: transcription, transcriptional, mediator, head, middle, tail, srb, rna polymerase ii, med
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 455878.23

構造登録者

Tsai, K.-L.,Yu, X.,Gopalan, S.,Chao, T.-C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F. (登録日: 2016-11-26, 公開日: 2017-03-08, 最終更新日: 2024-03-13)

主引用文献

Tsai, K.L.,Yu, X.,Gopalan, S.,Chao, T.C.,Zhang, Y.,Florens, L.,Washburn, M.P.,Murakami, K.,Conaway, R.C.,Conaway, J.W.,Asturias, F.J.
Mediator structure and rearrangements required for holoenzyme formation.
Nature, 544:196-201, 2017

PubMed Abstract: The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.

PubMed: 28241144
DOI: 10.1038/nature21393

実験手法

ELECTRON MICROSCOPY (4.4 Å)