5TVP

SUMO2 bound to Mouse Tdp2 catalytic domain with a 5'-phosphorylated DNA ternary complex

5TVP の概要

• エントリーDOI: 10.2210/pdb5tvp/pdb
• 関連するPDBエントリー: 1WM2 4GZ1 5TVQ
• 分子名称: Tyrosyl-DNA phosphodiesterase 2, DNA (5'-D(P*CP*CP*GP*AP*AP*TP*TP*CP*G)-3'), Small ubiquitin-related modifier 2, ... (7 entities in total)
• 機能のキーワード: hydrolase, dna repair, endonuclease/exonuclease/phosphatase (eep) domain, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 264101.73

構造登録者

Schellenberg, M.J.,Williams, R.S. (登録日: 2016-11-09, 公開日: 2017-10-11, 最終更新日: 2023-10-04)

主引用文献

Schellenberg, M.J.,Lieberman, J.A.,Herrero-Ruiz, A.,Butler, L.R.,Williams, J.G.,Munoz-Cabello, A.M.,Mueller, G.A.,London, R.E.,Cortes-Ledesma, F.,Williams, R.S.
ZATT (ZNF451)-mediated resolution of topoisomerase 2 DNA-protein cross-links.
Science, 357:1412-1416, 2017

PubMed Abstract: Topoisomerase 2 (TOP2) DNA transactions proceed via formation of the TOP2 cleavage complex (TOP2cc), a covalent enzyme-DNA reaction intermediate that is vulnerable to trapping by potent anticancer TOP2 drugs. How genotoxic TOP2 DNA-protein cross-links are resolved is unclear. We found that the SUMO (small ubiquitin-related modifier) ligase ZATT (ZNF451) is a multifunctional DNA repair factor that controls cellular responses to TOP2 damage. ZATT binding to TOP2cc facilitates a proteasome-independent tyrosyl-DNA phosphodiesterase 2 (TDP2) hydrolase activity on stalled TOP2cc. The ZATT SUMO ligase activity further promotes TDP2 interactions with SUMOylated TOP2, regulating efficient TDP2 recruitment through a "split-SIM" SUMO2 engagement platform. These findings uncover a ZATT-TDP2-catalyzed and SUMO2-modulated pathway for direct resolution of TOP2cc.

PubMed: 28912134
DOI: 10.1126/science.aam6468

実験手法

X-RAY DIFFRACTION (2.399 Å)