5TMA

Zymomonas mobilis pyruvate decarboxylase mutant PDC-2.3

Summary for 5TMA

DescriptorPyruvate decarboxylase, THIAMINE DIPHOSPHATE, MAGNESIUM ION, ... (6 entities in total)
Functional Keywordspyruvate decarboxylase, pdc, mutant, engineered, thermostable, cell adhesion
Biological sourceZymomonas mobilis
Total number of polymer chains2
Total molecular weight128430.74
Authors
Alahuhta, P.M.,Lunin, V.V. (deposition date: 2016-10-12, release date: 2017-10-18, Last modification date: 2019-06-05)
Primary citation
Sammond, D.W.,Kastelowitz, N.,Donohoe, B.S.,Alahuhta, M.,Lunin, V.V.,Chung, D.,Sarai, N.S.,Yin, H.,Mittal, A.,Himmel, M.E.,Guss, A.M.,Bomble, Y.J.
An iterative computational design approach to increase the thermal endurance of a mesophilic enzyme.
Biotechnol Biofuels, 11:189-189, 2018
PubMed: 30002729 (PDB entries with the same primary citation)
DOI: 10.1186/s13068-018-1178-9
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.67 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2096 0.3% 1.0% 2.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution