5TMA

Zymomonas mobilis pyruvate decarboxylase mutant PDC-2.3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Pyruvate decarboxylase	polymer	576	62438.6	2	UniProt (P06672) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) In PDB	Zymomonas mobilis	PDC
2	A, B	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	B, A	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
5	B, A	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
6		water	water		18.0	1004	Chemie (HOH)

Sequence modifications

A, B: 1 - 568 (UniProt: P06672)

PDB	External Database	Details
Asp 2	Ser 2	engineered mutation
Asp 38	Leu 38	engineered mutation
Ala 109	Gly 109	engineered mutation
Glu 207	Ala 207	engineered mutation
Ala 224	Gly 224	engineered mutation
Glu 333	Gln 333	engineered mutation
Asp 357	Ala 357	engineered mutation
Ile 374	Val 374	engineered mutation
Asp 376	Ala 376	engineered mutation
Ala 491	Gly 491	engineered mutation
Ala 515	Gly 515	engineered mutation
Ala 516	Gly 516	engineered mutation
Glu 519	Ala 519	engineered mutation
Asp 527	Ala 527	engineered mutation
Ala 540	Gly 540	engineered mutation
Glu 553	Lys 553	engineered mutation
Leu 569	-	expression tag
Glu 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	124877.3
Non-Polymers*	Number of molecules	38
	Total formula weight	3553.5
All*	Total formula weight	128430.7

*Water molecules are not included.