5TDH

The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S

Summary for 5TDH

• Entry DOI: 10.2210/pdb5tdh/pdb
• Descriptor: Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (4 entities in total)
• Functional Keywords: dominant negative, g-alpha(i)-1-beta-1-gamma-2 heterotrimer, g203a, a326s, gpcr, gdp, cell cycle
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : P63096; Cell membrane ; Lipid-anchor ; Cytoplasmic side : P63212
• Total number of polymer chains: 6
• Total formula weight: 172022.18

Authors

Liu, P.,Jia, M.-Z.,Zhou, X.E.,de Waal, P.W.,Dickson, B.M.,Liu, B.,Hou, L.,Yin, Y.-T.,Kang, Y.-Y.,Shi, Y.,Melcher, K.,Xu, H.E.,Jiang, Y. (deposition date: 2016-09-19, release date: 2016-11-09, Last modification date: 2024-03-20)

Primary citation

Liu, P.,Jia, M.-Z.,Zhou, X.E.,De Waal, P.W.,Dickson, B.M.,Liu, B.,Hou, L.,Yin, Y.-T.,Kang, Y.-Y.,Shi, Y.,Melcher, K.,Xu, H.E.,Jiang, Y.
The structural basis of the dominant negative phenotype of the G alpha i1 beta 1 gamma 2 G203A/A326S heterotrimer
Acta Pharmacol.Sin., 37:1259-1272, 2016

PubMed Abstract: Dominant negative mutant G proteins have provided critical insight into the mechanisms of G protein-coupled receptor (GPCR) signaling, but the mechanisms underlying the dominant negative characteristics are not completely understood. The aim of this study was to determine the structure of the dominant negative Gαi1β1γ2 G203A/A326S complex (Gi-DN) and to reveal the structural basis of the mutation-induced phenotype of Gαi1β1γ2.

PubMed: 27498775
DOI: 10.1038/aps.2016.69

Experimental method

X-RAY DIFFRACTION (3 Å)