5TDH

The crystal structure of the dominant negative mutant G protein alpha(i)-1-beta-1-gamma-2 G203A/A326S

5TDH の概要

• エントリーDOI: 10.2210/pdb5tdh/pdb
• 分子名称: Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (4 entities in total)
• 機能のキーワード: dominant negative, g-alpha(i)-1-beta-1-gamma-2 heterotrimer, g203a, a326s, gpcr, gdp, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : P63096; Cell membrane ; Lipid-anchor ; Cytoplasmic side : P63212
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 172022.18

構造登録者

Liu, P.,Jia, M.-Z.,Zhou, X.E.,de Waal, P.W.,Dickson, B.M.,Liu, B.,Hou, L.,Yin, Y.-T.,Kang, Y.-Y.,Shi, Y.,Melcher, K.,Xu, H.E.,Jiang, Y. (登録日: 2016-09-19, 公開日: 2016-11-09, 最終更新日: 2024-03-20)

主引用文献

Liu, P.,Jia, M.-Z.,Zhou, X.E.,De Waal, P.W.,Dickson, B.M.,Liu, B.,Hou, L.,Yin, Y.-T.,Kang, Y.-Y.,Shi, Y.,Melcher, K.,Xu, H.E.,Jiang, Y.
The structural basis of the dominant negative phenotype of the G alpha i1 beta 1 gamma 2 G203A/A326S heterotrimer
Acta Pharmacol.Sin., 37:1259-1272, 2016

PubMed Abstract: Dominant negative mutant G proteins have provided critical insight into the mechanisms of G protein-coupled receptor (GPCR) signaling, but the mechanisms underlying the dominant negative characteristics are not completely understood. The aim of this study was to determine the structure of the dominant negative Gαi1β1γ2 G203A/A326S complex (Gi-DN) and to reveal the structural basis of the mutation-induced phenotype of Gαi1β1γ2.

PubMed: 27498775
DOI: 10.1038/aps.2016.69

実験手法

X-RAY DIFFRACTION (3 Å)