5T9J

Crystal Structure of human GEN1 in complex with Holliday junction DNA in the upper interface

Summary for 5T9J

• Entry DOI: 10.2210/pdb5t9j/pdb
• Descriptor: Flap endonuclease GEN homolog 1, DNA (5'-D(*DGP*DAP*DAP*DTP*DTP*DCP*DCP*DGP*DGP*DAP*DTP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DGP*DC)-3'), DNA (5'-D(*DGP*DCP*DAP*DTP*DCP*DCP*DCP*DTP*DAP*DAP*DGP*DCP*DTP*DCP*DCP*DAP*DTP*DCP*DGP*DT)-3'), ... (8 entities in total)
• Functional Keywords: protein-dna complex, holliday junction resolvase, structure-specific endonuclease, dna four-way junction, hydrolase
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q17RS7
• Total number of polymer chains: 6
• Total formula weight: 142758.05

Authors

Lee, S.-H.,Biertumpfel, C. (deposition date: 2016-09-09, release date: 2016-09-21, Last modification date: 2024-05-08)

Primary citation

Lee, S.H.,Princz, L.N.,Klugel, M.F.,Habermann, B.,Pfander, B.,Biertumpfel, C.
Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage.
Elife, 4:-, 2015

PubMed Abstract: Holliday junctions (HJs) are key DNA intermediates in homologous recombination. They link homologous DNA strands and have to be faithfully removed for proper DNA segregation and genome integrity. Here, we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 Å resolution. The GEN1 core is similar to other Rad2/XPG nucleases. However, unlike other members of the superfamily, GEN1 contains a chromodomain as an additional DNA interaction site. Chromodomains are known for their chromatin-targeting function in chromatin remodelers and histone(de)acetylases but they have not previously been found in nucleases. The GEN1 chromodomain directly contacts DNA and its truncation severely hampers GEN1's catalytic activity. Structure-guided mutations in vitro and in vivo in yeast validated our mechanistic findings. Our study provides the missing structure in the Rad2/XPG family and insights how a well-conserved nuclease core acquires versatility in recognizing diverse substrates for DNA repair and maintenance.

PubMed: 26682650
DOI: 10.7554/eLife.12256

Experimental method

X-RAY DIFFRACTION (3.00012743215 Å)