5T9J
Crystal Structure of human GEN1 in complex with Holliday junction DNA in the upper interface
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Flap endonuclease GEN homolog 1 | polymer | 506 | 58394.6 | 2 | UniProt (Q17RS7) Pfam (PF00752) Pfam (PF00867) Pfam (PF18704) | Homo sapiens (Human) | |
| 2 | C (C) | DNA (5'-D(*DGP*DAP*DAP*DTP*DTP*DCP*DCP*DGP*DGP*DAP*DTP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DGP*DC)-3') | polymer | 20 | 6135.0 | 1 | synthetic construct | ||
| 3 | D (D) | DNA (5'-D(*DGP*DCP*DAP*DTP*DCP*DCP*DCP*DTP*DAP*DAP*DGP*DCP*DTP*DCP*DCP*DAP*DTP*DCP*DGP*DT)-3') | polymer | 20 | 6160.0 | 1 | synthetic construct | ||
| 4 | E (E) | DNA (5'-D(*DAP*DCP*DGP*DAP*DTP*DGP*DGP*DAP*DGP*DCP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DCP*DTP*DC)-3') | polymer | 20 | 6029.9 | 1 | synthetic construct | ||
| 5 | F (F) | DNA (5'-D(*DGP*DAP*DGP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DTP*DCP*DCP*DGP*DGP*DAP*DAP*DTP*DTP*DC)-3') | polymer | 20 | 6214.0 | 1 | synthetic construct | ||
| 6 | G, H, I, J, K... (A, B, C) | GLYCEROL | non-polymer | 92.1 | 15 | Chemie (GOL) | |||
| 7 | N, T (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | AA, X, Y, Z (F, A, B, D) | water | water | 18.0 | 23 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 505 (UniProt: Q17RS7)
| PDB | External Database | Details |
|---|---|---|
| Asn 30 | Asp 30 | engineered mutation |
| Thr 92 | Ser 92 | variant |
| Asn 310 | Ser 310 | variant |
| Leu 506 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 141328.0 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 1430.0 | |
| All* | Total formula weight | 142758.0 |
