5T8D
Engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 47 point mutations relative to wild-type I-OnuI
Summary for 5T8D
Entry DOI | 10.2210/pdb5t8d/pdb |
Related | 5V0Q |
Descriptor | I-OnuI_e-vHIVInt_v2, DNA (26-MER), CALCIUM ION, ... (5 entities in total) |
Functional Keywords | meganuclease, engineered protein, dna complex, homing endonuclease, hydrolase-dna complex, hydrolase/dna |
Biological source | synthetic construct More |
Total number of polymer chains | 3 |
Total formula weight | 50521.32 |
Authors | Stoddard, B.L.,Werther, R.,Lambert, A.R. (deposition date: 2016-09-07, release date: 2017-05-03, Last modification date: 2023-10-04) |
Primary citation | Niyonzima, N.,Lambert, A.R.,Werther, R.,De Silva Feelixge, H.,Roychoudhury, P.,Greninger, A.L.,Stone, D.,Stoddard, B.L.,Jerome, K.R. Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses. Protein Eng.Des.Sel., 30:503-522, 2017 Cited by PubMed: 28873986DOI: 10.1093/protein/gzx037 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.15 Å) |
Structure validation
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