5T2O

Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP011377 gene; harbors 53 point mutations relative to wild-type I-OnuI

Summary for 5T2O

• Entry DOI: 10.2210/pdb5t2o/pdb
• Related: 5T2H 5T2J 5T2N
• Descriptor: I-OnuI_e-ag011377, DNA (26-MER), CALCIUM ION, ... (5 entities in total)
• Functional Keywords: meganuclease, engineered protein, dna complex, homing endonuclease, hydrolase-dna complex, hydrolase/dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 3
• Total formula weight: 50948.86

Authors

Stoddard, B.L.,Werther, R. (deposition date: 2016-08-23, release date: 2017-05-03, Last modification date: 2023-10-04)

• Primary citation: Werther, R.,Hallinan, J.P.,Lambert, A.R.,Havens, K.,Pogson, M.,Jarjour, J.,Galizi, R.,Windbichler, N.,Crisanti, A.,Nolan, T.,Stoddard, B.L.; Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.; Nucleic Acids Res., 45:8621-8634, 2017; PubMed: 28637173; DOI: 10.1093/nar/gkx544

Experimental method

X-RAY DIFFRACTION (2.801 Å)