Mediator-RNA Polymerase II Pre-Initiation Complex

Summary for 5SVA

EMDB information8305 8307 8308
DescriptorDNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (42 entities in total)
Functional Keywordstranscriptional initiation, mediator, pre-initiation complex, carboxy-terminal domain (ctd), transcription, transferase-dna complex, transferase/dna
Biological sourceSaccharomyces cerevisiae (Baker's yeast)
Cellular locationNucleus P04050 P08518 P16370 P20433 P20434 P34087 P20436 P38902 P38782 P38304 Q99278 P32569 P32585 P34162 P32570 Q12343 Q08278 P33308 P47822 P38633 P06839 Q00578 Q02939 Q3E7C1 P29055 P41895 P41896 P36100 P36145 P13393 P04050 P35189
Cytoplasm  P20435 P32773 P32774
Nucleus, nucleolus  P27999 P22139 P40422
Total number of polymer chains40
Total molecular weight1522751.47
Robinson, P.J.,Bushnell, D.A.,Kornberg, R.D. (deposition date: 2016-08-05, release date: 2016-09-28, Last modification date: 2017-09-13)
Primary citation
Robinson, P.J.,Trnka, M.J.,Bushnell, D.A.,Davis, R.E.,Mattei, P.J.,Burlingame, A.L.,Kornberg, R.D.
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Cell, 166:1411-1422.e16, 2016
PubMed: 27610567 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2016.08.050
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliers503.2%9.7%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures