5SVA
Mediator-RNA Polymerase II Pre-Initiation Complex
Summary for 5SVA
| Entry DOI | 10.2210/pdb5sva/pdb |
| EMDB information | 8305 8307 8308 |
| Descriptor | DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (42 entities in total) |
| Functional Keywords | transcriptional initiation, mediator, pre-initiation complex, carboxy-terminal domain (ctd), transcription, transferase-dna complex, transferase/dna |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) More |
| Total number of polymer chains | 40 |
| Total formula weight | 1522751.47 |
| Authors | Robinson, P.J.,Bushnell, D.A.,Kornberg, R.D. (deposition date: 2016-08-05, release date: 2016-09-28, Last modification date: 2019-11-27) |
| Primary citation | Robinson, P.J.,Trnka, M.J.,Bushnell, D.A.,Davis, R.E.,Mattei, P.J.,Burlingame, A.L.,Kornberg, R.D. Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex. Cell, 166:1411-1422.e16, 2016 Cited by PubMed Abstract: A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription. PubMed: 27610567DOI: 10.1016/j.cell.2016.08.050 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (15.3 Å) |
Structure validation
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