5OY7

Structure of the 4_601_157 tetranucleosome (P1 form)

Summary for 5OY7

• Entry DOI: 10.2210/pdb5oy7/pdb
• Related: 5OXV
• Descriptor: Histone H2B 1.1, Histone H3, Histone H4, ... (7 entities in total)
• Functional Keywords: nucleosome, histone, dna, tetranucleosome, chromatin fiber, gene regulation
• Biological source: Xenopus laevis (African clawed frog); More
• Total number of polymer chains: 34
• Total formula weight: 827371.85

Authors

Ekundayo, B.,Richmond, T.J.,Schalch, T. (deposition date: 2017-09-07, release date: 2017-09-20, Last modification date: 2024-01-17)

Primary citation

Ekundayo, B.,Richmond, T.J.,Schalch, T.
Capturing Structural Heterogeneity in Chromatin Fibers.
J. Mol. Biol., 429:3031-3042, 2017

PubMed Abstract: Chromatin fiber organization is implicated in processes such as transcription, DNA repair and chromosome segregation, but how nucleosomes interact to form higher-order structure remains poorly understood. We solved two crystal structures of tetranucleosomes with approximately 11-bp DNA linker length at 5.8 and 6.7 Å resolution. Minimal intramolecular nucleosome-nucleosome interactions result in a fiber model resembling a flat ribbon that is compatible with a two-start helical architecture, and that exposes histone and DNA surfaces to the environment. The differences in the two structures combined with electron microscopy reveal heterogeneous structural states, and we used site-specific chemical crosslinking to assess the diversity of nucleosome-nucleosome interactions through identification of structure-sensitive crosslink sites that provide a means to characterize fibers in solution. The chromatin fiber architectures observed here provide a basis for understanding heterogeneous chromatin higher-order structures as they occur in a genomic context.

PubMed: 28893533
DOI: 10.1016/j.jmb.2017.09.002

Experimental method

X-RAY DIFFRACTION (5.774 Å)