5OY7

Structure of the 4_601_157 tetranucleosome (P1 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-25
Detector	MAR CCD 165 mm
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	66.624, 161.465, 227.915
Unit cell angles	78.94, 83.86, 83.74

Refinement procedure

Resolution	111.407 - 5.774
R-factor	0.2199
Rwork	0.219
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.009
RMSD bond angle	1.462
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.500
High resolution limit [Å]	5.770	5.770
Rmerge	0.110
Rmeas	0.076	0.506
Number of reflections	24331	4652
<I/σ(I)>	4.9	1.63
Completeness [%]	93.8	82
Redundancy	1.9
CC(1/2)	0.996	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	30-60 mM KCl, 90-110 mM MgCl2 and 5 mM Na-cacodyalte, pH 6.0