5OFA

Crystal structure of human MORC2 (residues 1-603) with spinal muscular atrophy mutation T424R

Summary for 5OFA

• Entry DOI: 10.2210/pdb5ofa/pdb
• Descriptor: MORC family CW-type zinc finger protein 2, ZINC ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• Functional Keywords: ghkl atpase, chromatin remodeler, epigenetic silencing, transcriptional repressor, coiled-coil, cw domain, dna binding protein, charcot-marie-tooth disease, spinal muscular atrophy, nuclear protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 141342.01

Authors

Douse, C.H.,Liu, Y.,Modis, Y. (deposition date: 2017-07-10, release date: 2018-02-14, Last modification date: 2024-05-01)

Primary citation

Douse, C.H.,Bloor, S.,Liu, Y.,Shamin, M.,Tchasovnikarova, I.A.,Timms, R.T.,Lehner, P.J.,Modis, Y.
Neuropathic MORC2 mutations perturb GHKL ATPase dimerization dynamics and epigenetic silencing by multiple structural mechanisms.
Nat Commun, 9:651-651, 2018

PubMed Abstract: Missense mutations in MORC2 cause neuropathies including spinal muscular atrophy and Charcot-Marie-Tooth disease. We recently identified MORC2 as an effector of epigenetic silencing by the human silencing hub (HUSH). Here we report the biochemical and cellular activities of MORC2 variants, alongside crystal structures of wild-type and neuropathic forms of a human MORC2 fragment comprising the GHKL-type ATPase module and CW-type zinc finger. This fragment dimerizes upon binding ATP and contains a hinged, functionally critical coiled-coil insertion absent in other GHKL ATPases. We find that dimerization and DNA binding of the MORC2 ATPase module transduce HUSH-dependent silencing. Disease mutations change the dynamics of dimerization by distinct structural mechanisms: destabilizing the ATPase-CW module, trapping the ATP lid, or perturbing the dimer interface. These defects lead to the modulation of HUSH function, thus providing a molecular basis for understanding MORC2-associated neuropathies.

PubMed: 29440755
DOI: 10.1038/s41467-018-03045-x

Experimental method

X-RAY DIFFRACTION (2.57 Å)