5NKL

Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dDs-dPxTP

Summary for 5NKL

• Entry DOI: 10.2210/pdb5nkl/pdb
• Descriptor: DNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), DNA (5'-D(*AP*AP*AP*(DNU)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), ... (9 entities in total)
• Functional Keywords: dna polymerase, artificial base pair, unnatural base pair, ternary complex, transferase
• Biological source: Thermus aquaticus; More
• Total number of polymer chains: 3
• Total formula weight: 70993.00

Authors

Betz, K.,Marx, A.,Diederichs, K.,Hirao, I.,Kimoto, M. (deposition date: 2017-03-31, release date: 2017-06-28, Last modification date: 2024-01-17)

• Primary citation: Betz, K.,Kimoto, M.,Diederichs, K.,Hirao, I.,Marx, A.; Structural Basis for Expansion of the Genetic Alphabet with an Artificial Nucleobase Pair.; Angew. Chem. Int. Ed. Engl., 56:12000-12003, 2017; PubMed: 28594080; DOI: 10.1002/anie.201704190

Experimental method

X-RAY DIFFRACTION (1.7 Å)