5NKL
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dDs-dPxTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I, thermostable | polymer | 540 | 60937.0 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) | Thermus aquaticus | Taq polymerase 1 |
| 2 | B (B) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3') | polymer | 12 | 3617.4 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (5'-D(*AP*AP*AP*(DNU)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 16 | 5014.3 | 1 | synthetic construct | ||
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 6 | G (A) | [[(2~{R},3~{S},5~{R})-5-[4-[(4~{R})-4,5-bis(oxidanyl)pent-1-ynyl]-2-nitro-pyrrol-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate | non-polymer | 566.2 | 1 | Chemie (91T) | |||
| 7 | H (A) | [[(2~{R},3~{S},5~{R})-5-[4-[(4~{S})-4,5-bis(oxidanyl)pent-1-ynyl]-2-nitro-pyrrol-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate | non-polymer | 566.2 | 1 | Chemie (91R) | |||
| 8 | I, J (C) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 9 | K, L, M (A, B, C) | water | water | 18.0 | 303 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 69568.7 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1424.3 | |
| All* | Total formula weight | 70993.0 |
