5MRF

Structure of the yeast mitochondrial ribosome - Class C

This is a non-PDB format compatible entry.

Summary for 5MRF

• Entry DOI: 10.2210/pdb5mrf/pdb
• EMDB information: 2566 3553
• Descriptor: 21S ribosomal RNA, uL15m, uL16m, ... (82 entities in total)
• Functional Keywords: yeast, mitochondrial, ribosome, saccharomyces cerevisiae
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Total number of polymer chains: 78
• Total formula weight: 3325585.75

Authors

Desai, N.,Brown, A.,Amunts, A.,Ramakrishnan, V. (deposition date: 2016-12-22, release date: 2017-02-15, Last modification date: 2024-05-15)

Primary citation

Desai, N.,Brown, A.,Amunts, A.,Ramakrishnan, V.
The structure of the yeast mitochondrial ribosome.
Science, 355:528-531, 2017

PubMed Abstract: Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation.

PubMed: 28154081
DOI: 10.1126/science.aal2415

Experimental method

ELECTRON MICROSCOPY (4.97 Å)