5MGD

STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose

Summary for 5MGD

• Entry DOI: 10.2210/pdb5mgd/pdb
• Related: 5IFP 5IFT 5IHR 5JUV 5MGC
• Descriptor: Probable beta-galactosidase A, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• Functional Keywords: tim barrel, gh35, glycoside hydrolase, kinetics, protein conformation, carbohydrate metabolism, b-galactosidase, aspergillus niger, fungal protein, substrate specificity, prebiotics, galactooligosaccharides, gos, recombinant, 6-gal-lac, 6-galactosyllactose, hydrolase
• Biological source: Aspergillus niger CBS 513.88
• Cellular location: Secreted : A2QAN3
• Total number of polymer chains: 1
• Total formula weight: 117003.15

Authors

Rico-Diaz, A.,Ramirez-Escudero, M.,Vizoso Vazquez, A.,Cerdan, M.E.,Becerra, M.,Sanz-Aparicio, J. (deposition date: 2016-11-21, release date: 2017-04-19, Last modification date: 2024-01-17)

• Primary citation: Rico-Diaz, A.,Ramirez-Escudero, M.,Vizoso-Vazquez, A.,Cerdan, M.E.,Becerra, M.,Sanz-Aparicio, J.; Structural features of Aspergillus niger beta-galactosidase define its activity against glycoside linkages.; FEBS J., 284:1815-1829, 2017; PubMed: 28391618; DOI: 10.1111/febs.14083

Experimental method

X-RAY DIFFRACTION (2.15 Å)