5IHR

STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH ALLOLACTOSE

Summary for 5IHR

• Entry DOI: 10.2210/pdb5ihr/pdb
• Related: 5IFP 5IFT
• Descriptor: Probable beta-galactosidase A, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: tim barrel, gh35, glycoside hydrolase, kinetics, protein conformation, carbohydrate metabolism, b-galactosidase, aspergillus niger, fungal protein, substrate specificity, prebiotics, galactooligosaccharides, gos, recombinant, allolactose, b-d-galactopyranosyl-(1-6)-d-glucopyranose, hydrolase
• Biological source: Aspergillus niger CBS 513.88
• Cellular location: Secreted : A2QAN3
• Total number of polymer chains: 1
• Total formula weight: 116999.57

Authors

Rico-Diaz, A.,Ramirez-Escudero, M.,Vizoso Vazquez, A.,Cerdan, M.E.,Becerra, M.,Sanz-Aparicio, J. (deposition date: 2016-02-29, release date: 2017-04-19, Last modification date: 2024-01-10)

• Primary citation: Rico-Diaz, A.,Ramirez-Escudero, M.,Vizoso-Vazquez, A.,Cerdan, M.E.,Becerra, M.,Sanz-Aparicio, J.; Structural features of Aspergillus niger beta-galactosidase define its activity against glycoside linkages.; FEBS J., 284:1815-1829, 2017; PubMed: 28391618; DOI: 10.1111/febs.14083

Experimental method

X-RAY DIFFRACTION (2.4 Å)