5IHR

STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH ALLOLACTOSE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Probable beta-galactosidase A	polymer	1013	110627.9	1	UniProt (A2QAN3) Pfam (PF01301) Pfam (PF10435) Pfam (PF13363) Pfam (PF13364) In PDB	Aspergillus niger CBS 513.88	Lactase A
2	B, C	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	2	In PDB GlyTouCan (G83582BK)
3	D	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G01760ZU)
4	E	beta-D-galactopyranose-(1-6)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G49736TE)
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
6	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	A	DIMETHYL SULFOXIDE	non-polymer		78.1	9	Chemie (DMS)
9		water	water		18.0	266	Chemie (HOH)

Sequence modifications

A: 1 - 1007 (UniProt: A2QAN3)

PDB	External Database	Details
Gln 298	Glu 298	engineered mutation
His 1008	-	expression tag
His 1009	-	expression tag
His 1010	-	expression tag
His 1011	-	expression tag
His 1012	-	expression tag
His 1013	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	110627.9
Branched	Number of molecules	4
	Total formula weight	4209.8
Non-Polymers*	Number of molecules	17
	Total formula weight	2162.0
All*	Total formula weight	116999.6

*Water molecules are not included.