5MGD
STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Probable beta-galactosidase A | polymer | 1013 | 110627.9 | 1 | UniProt (A2QAN3) Pfam (PF01301) Pfam (PF10435) Pfam (PF13363) Pfam (PF13364) In PDB | Aspergillus niger CBS 513.88 | Lactase A |
| 2 | B | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G83582BK) | |||
| 3 | C | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 4 | D | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G01760ZU) | |||
| 5 | E | beta-D-galactopyranose-(1-6)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G78961SJ) | |||
| 6 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
| 7 | A | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 1 | Chemie (DMS) | |||
| 8 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 9 | A | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 10 | water | water | 18.0 | 185 | Chemie (HOH) |
Sequence modifications
A: 1 - 1007 (UniProt: A2QAN3)
| PDB | External Database | Details |
|---|---|---|
| Gln 298 | Glu 298 | engineered mutation |
| His 1008 | - | expression tag |
| His 1009 | - | expression tag |
| His 1010 | - | expression tag |
| His 1011 | - | expression tag |
| His 1012 | - | expression tag |
| His 1013 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 110627.9 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4696.2 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1679.1 | |
| All* | Total formula weight | 117003.1 |
