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5M5C

Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins

Summary for 5M5C
Entry DOI10.2210/pdb5m5c/pdb
Related5M50 5M54
EMDB information3444 4154 4156
DescriptorCalmodulin-regulated spectrin-associated protein 1, Tubulin alpha chain, Tubulin beta-2B chain, ... (8 entities in total)
Functional Keywordscamsap ckk microtubule tubulin, transport protein
Biological sourceHomo sapiens (Human)
More
Cellular locationCytoplasm, cytoskeleton : Q5T5Y3 F2Z4C1 Q6B856
Total number of polymer chains5
Total formula weight210136.92
Authors
Primary citationAtherton, J.,Jiang, K.,Stangier, M.M.,Luo, Y.,Hua, S.,Houben, K.,van Hooff, J.J.E.,Joseph, A.P.,Scarabelli, G.,Grant, B.J.,Roberts, A.J.,Topf, M.,Steinmetz, M.O.,Baldus, M.,Moores, C.A.,Akhmanova, A.
A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
Nat. Struct. Mol. Biol., 24:931-943, 2017
Cited by
PubMed Abstract: CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we found that the CAMSAP C-terminal CKK domain is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncovered how mammalian CKK binds between two tubulin dimers at the interprotofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high-resolution fluorescence microscopy and cryo-electron tomography suggested that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the interprotofilament interface at this site serve as the basis for CKK's minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends.
PubMed: 28991265
DOI: 10.1038/nsmb.3483
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (4.8 Å)
Structure validation

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