5M32
Human 26S proteasome in complex with Oprozomib
Summary for 5M32
| Entry DOI | 10.2210/pdb5m32/pdb |
| EMDB information | 4146 |
| Descriptor | Proteasome subunit alpha type-2, Proteasome subunit beta type-2, Proteasome subunit beta type-5, ... (35 entities in total) |
| Functional Keywords | proteasome, oprozomib, ups, drug-binding, hydrolase |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 47 |
| Total formula weight | 1535198.08 |
| Authors | Haselbach, D.,Schrader, J.,Lambrecht, F.,Henneberg, F.,Chari, A.,Stark, H. (deposition date: 2016-10-14, release date: 2017-07-05, Last modification date: 2025-04-09) |
| Primary citation | Haselbach, D.,Schrader, J.,Lambrecht, F.,Henneberg, F.,Chari, A.,Stark, H. Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs. Nat Commun, 8:15578-15578, 2017 Cited by PubMed Abstract: The proteasome holoenzyme is the major non-lysosomal protease; its proteolytic activity is essential for cellular homeostasis. Thus, it is an attractive target for the development of chemotherapeutics. While the structural basis of core particle (CP) inhibitors is largely understood, their structural impact on the proteasome holoenzyme remains entirely elusive. Here, we determined the structure of the 26S proteasome with and without the inhibitor Oprozomib. Drug binding modifies the energy landscape of conformational motion in the proteasome regulatory particle (RP). Structurally, the energy barrier created by Oprozomib triggers a long-range allosteric regulation, resulting in the stabilization of a non-productive state. Thereby, the chemical drug-binding signal is converted, propagated and amplified into structural changes over a distance of more than 150 Å from the proteolytic site to the ubiquitin receptor Rpn10. The direct visualization of changes in conformational dynamics upon drug binding allows new ways to screen and develop future allosteric proteasome inhibitors. PubMed: 28541292DOI: 10.1038/ncomms15578 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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