5M32
Human 26S proteasome in complex with Oprozomib
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, O (A, O) | Proteasome subunit alpha type-2 | polymer | 234 | 25927.5 | 2 | UniProt (P25787) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3 |
| 2 | B, P (B, P) | Proteasome subunit alpha type-4 | polymer | 261 | 29525.8 | 2 | UniProt (P25789) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L |
| 3 | C (C) | Proteasome subunit alpha type-7 | polymer | 234 | 26164.7 | 1 | UniProt (O14818) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Proteasome subunit RC6-1,Proteasome subunit XAPC7 |
| 4 | D, R (D, R) | Proteasome subunit alpha type-5 | polymer | 241 | 26436.0 | 2 | UniProt (P28066) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome zeta chain |
| 5 | E (E) | Proteasome subunit alpha type-1 | polymer | 234 | 26203.8 | 1 | UniProt (P25786) Pfam (PF00227) Pfam (PF10584) | Homo sapiens (Human) | 30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain |
| 6 | F, T (F, T) | Proteasome subunit alpha type-3 | polymer | 255 | 28469.3 | 2 | UniProt (P25788) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8 |
| 7 | G, U (G, U) | Proteasome subunit alpha type-6 | polymer | 246 | 27432.5 | 2 | UniProt (P60900) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain |
| 8 | H, V (H, V) | Proteasome subunit beta type-7 | polymer | 277 | 30000.4 | 2 | UniProt (Q99436) Pfam (PF00227) Pfam (PF12465) | Homo sapiens (Human) | Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z |
| 9 | I, W (I, W) | Proteasome subunit beta type-3 | polymer | 205 | 22972.9 | 2 | UniProt (P49720) Pfam (PF00227) | Homo sapiens (Human) | Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain |
| 10 | J, X (J, X) | Proteasome subunit beta type-2 | polymer | 196 | 22349.7 | 2 | UniProt (P49721) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C7-I,Multicatalytic endopeptidase complex subunit C7-I,Proteasome component C7-I |
| 11 | K, Y (K, Y) | Proteasome subunit beta type-5 | polymer | 204 | 22484.4 | 2 | UniProt (P28074) Pfam (PF00227) | Homo sapiens (Human) | Macropain epsilon chain,Multicatalytic endopeptidase complex epsilon chain,Proteasome chain 6,Proteasome epsilon chain,Proteasome subunit MB1,Proteasome subunit X |
| 12 | L, Z (L, Z) | Proteasome subunit beta type-1 | polymer | 241 | 26522.4 | 2 | UniProt (P20618) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain |
| 13 | AA, M (M, a) | Proteasome subunit beta type-4 | polymer | 264 | 29231.2 | 2 | UniProt (P28070) Pfam (PF00227) | Homo sapiens (Human) | 26 kDa prosomal protein,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3 |
| 14 | BA, N (N, b) | Proteasome subunit beta type-6 | polymer | 239 | 25377.7 | 2 | UniProt (P28072) Pfam (PF00227) | Homo sapiens (Human) | Macropain delta chain,Multicatalytic endopeptidase complex delta chain,Proteasome delta chain,Proteasome subunit Y |
| 15 | Q (Q) | Proteasome subunit alpha type-7 | polymer | 235 | 26351.0 | 1 | UniProt (O14818) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Proteasome subunit RC6-1,Proteasome subunit XAPC7 |
| 16 | S (S) | Proteasome subunit alpha type-1 | polymer | 238 | 26632.3 | 1 | UniProt (P25786) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain |
| 17 | CA (c) | 26S protease regulatory subunit 7 | polymer | 433 | 48700.8 | 1 | UniProt (P35998) Pfam (PF21236) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2,Protein MSS1 |
| 18 | DA (d) | 26S protease regulatory subunit 4 | polymer | 428 | 47914.0 | 1 | UniProt (P62191) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1 |
| 19 | EA (e) | 26S protease regulatory subunit 6B | polymer | 418 | 47426.1 | 1 | UniProt (P43686) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7 |
| 20 | FA (f) | 26S protease regulatory subunit 10B | polymer | 379 | 43065.6 | 1 | UniProt (P62333) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT4,Proteasome 26S subunit ATPase 6,Proteasome subunit p42 |
| 21 | GA (g) | 26S protease regulatory subunit 6A | polymer | 439 | 49266.5 | 1 | UniProt (P17980) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1 |
| 22 | HA (h) | 26S protease regulatory subunit 8 | polymer | 355 | 39818.3 | 1 | UniProt (P62195) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG |
| 23 | IA (i) | 26S proteasome non-ATPase regulatory subunit 1 | polymer | 953 | 105958.2 | 1 | UniProt (Q99460) Pfam (PF21505) Pfam (PF01851) Pfam (PF13646) Pfam (PF18004) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112 |
| 24 | JA (j) | 26S proteasome non-ATPase regulatory subunit 3 | polymer | 534 | 61066.5 | 1 | UniProt (O43242) Pfam (PF01399) Pfam (PF08375) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN3,26S proteasome regulatory subunit S3,Proteasome subunit p58 |
| 25 | KA (k) | 26S proteasome non-ATPase regulatory subunit 12 | polymer | 456 | 52979.4 | 1 | UniProt (O00232) Pfam (PF22241) Pfam (PF01399) Pfam (PF18098) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN5,26S proteasome regulatory subunit p55 |
| 26 | LA (l) | 26S proteasome non-ATPase regulatory subunit 11 | polymer | 422 | 47526.7 | 1 | UniProt (O00231) Pfam (PF18055) Pfam (PF01399) Pfam (PF18503) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN6,26S proteasome regulatory subunit S9,26S proteasome regulatory subunit p44.5 |
| 27 | MA (m) | 26S proteasome non-ATPase regulatory subunit 6 | polymer | 389 | 45592.3 | 1 | UniProt (Q15008) Pfam (PF10602) Pfam (PF01399) Pfam (PF21154) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN7,26S proteasome regulatory subunit S10,Breast cancer-associated protein SGA-113M,Phosphonoformate immuno-associated protein 4,Proteasome regulatory particle subunit p44S10,p42A |
| 28 | NA (n) | 26S proteasome non-ATPase regulatory subunit 7 | polymer | 324 | 37086.4 | 1 | UniProt (P51665) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN8,26S proteasome regulatory subunit S12,Mov34 protein homolog,Proteasome subunit p40 |
| 29 | OA (o) | 26S proteasome non-ATPase regulatory subunit 13 | polymer | 376 | 42995.4 | 1 | UniProt (Q9UNM6) Pfam (PF22037) Pfam (PF01399) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN9,26S proteasome regulatory subunit S11,26S proteasome regulatory subunit p40.5 |
| 30 | PA (p) | 26S proteasome non-ATPase regulatory subunit 4 | polymer | 377 | 40781.6 | 1 | UniProt (P55036) Pfam (PF13519) Pfam (PF02809) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Antisecretory factor 1,ASF,Multiubiquitin chain-binding protein |
| 31 | QA (q) | 26S proteasome non-ATPase regulatory subunit 14 | polymer | 310 | 34620.0 | 1 | UniProt (O00487) Pfam (PF01398) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN11,26S proteasome-associated PAD1 homolog 1 |
| 32 | RA (r) | 26S proteasome non-ATPase regulatory subunit 8 | polymer | 350 | 39667.9 | 1 | UniProt (P48556) Pfam (PF10075) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN12,26S proteasome regulatory subunit S14,p31 |
| 33 | SA (s) | 26S proteasome complex subunit SEM1 | polymer | 70 | 8284.6 | 1 | UniProt (P60896) Pfam (PF05160) | Homo sapiens (Human) | 26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein |
| 34 | TA, UA (t, u) | bound Oprozomib | polymer | 4 | 536.6 | 2 | Synthetic construct | ||
| 35 | AB, VA, WA, XA, YA... (h, c, d, e, g) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 6 | Chemie (ADP) |
Sequence modifications
C: 2 - 235 (UniProt: O14818)
E: 4 - 237 (UniProt: P25786)
J, X: 1 - 196 (UniProt: P49721)
Q: 2 - 236 (UniProt: O14818)
S: 2 - 239 (UniProt: P25786)
| PDB | External Database | Details |
|---|---|---|
| Ala 48 | Lys 48 | conflict |
| Asp 179 | Glu 179 | conflict |
| Glu 200 | Gln 200 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 185 | Asn 185 | conflict |
| Asp 234 | Glu 234 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 182 | Ile 182 | conflict |
| Asp 186 | Asn 186 | conflict |
| Asn 190 | Asp 190 | conflict |
| Glu 192 | Asp 192 | conflict |
| Ala 195 | Ser 195 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 179 | Glu 179 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | Phe 2 | conflict |
| Ala 3 | Arg 3 | conflict |
| Asp 185 | Asn 185 | conflict |
| Asp 234 | Glu 234 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 47 |
| Total formula weight | 1532634.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2563.2 | |
| All* | Total formula weight | 1535198.1 |
