5LGW

GlgE isoform 1 from Streptomyces coelicolor D394A mutant co-crystallised with maltodextrin

Summary for 5LGW

• Entry DOI: 10.2210/pdb5lgw/pdb
• Related PRD ID: PRD_900018 PRD_900030
• Descriptor: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-[alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)]alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (8 entities in total)
• Functional Keywords: hydrolase, alpha-glucan biosynthesis, glycoside hydrolase family 13_3, transferase
• Biological source: Streptomyces coelicolor
• Total number of polymer chains: 2
• Total formula weight: 160292.24

Authors

Syson, K.,Stevenson, C.E.M.,Mia, F.,Barclay, J.E.,Tang, M.,Gorelik, A.,Rashid, A.M.,Lawson, D.M.,Bornemann, S. (deposition date: 2016-07-08, release date: 2016-08-17, Last modification date: 2024-01-10)

• Primary citation: Syson, K.,Stevenson, C.E.,Miah, F.,Barclay, J.E.,Tang, M.,Gorelik, A.,Rashid, A.M.,Lawson, D.M.,Bornemann, S.; Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor maltosyltransferase GlgE.; J.Biol.Chem., 291:21531-21540, 2016; PubMed: 27531751; DOI: 10.1074/jbc.M116.748160

Experimental method

X-RAY DIFFRACTION (1.95 Å)