5LGW

GlgE isoform 1 from Streptomyces coelicolor D394A mutant co-crystallised with maltodextrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	115.110, 115.110, 311.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.400 - 1.950
R-factor	0.178
Rwork	0.177
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zst
RMSD bond length	0.010
RMSD bond angle	1.405
Data reduction software	XDS
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.400	81.400	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.083	0.036	0.915
Number of reflections	147828
<I/σ(I)>	17.5		1.2
Completeness [%]	97.1	99.4	79.9
Redundancy	13.1	15.6	3.1
CC(1/2)	0.999	0.999	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NULL