5LGW
GlgE isoform 1 from Streptomyces coelicolor D394A mutant co-crystallised with maltodextrin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1 | polymer | 695 | 77520.8 | 2 | UniProt (Q9L1K2) Pfam (PF11896) Pfam (PF21702) | Streptomyces coelicolor | GMPMT 1,(1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase 1 |
| 2 | C, E (C, E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 2 | In PDB BIRD (PRD_900030) GlyTouCan (G50146AM) | alpha-maltopentaose | ||
| 3 | D (D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-[alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)]alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1477.3 | 1 | In PDB GlyTouCan (G57324CH) | |||
| 4 | F (F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 990.9 | 1 | In PDB GlyTouCan (G46758UT) | |||
| 5 | G, H (G, H) | alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900018) GlyTouCan (G66120GK) | beta-maltose | ||
| 6 | I, J, L, M (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 7 | K (A) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 8 | N, O (A, B) | water | water | 18.0 | 1092 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 675 (UniProt: Q9L1K2)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 394 | Asp 394 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 155041.7 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 4810.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 440.4 | |
| All* | Total formula weight | 160292.2 |
