5LEF

Rab6A:Kif20A complex

Summary for 5LEF

• Entry DOI: 10.2210/pdb5lef/pdb
• Descriptor: Ras-related protein Rab-6A, Kinesin-like protein KIF20A, GUANOSINE-5'-TRIPHOSPHATE, ... (8 entities in total)
• Functional Keywords: rab gtpase, kinesin, motor, protein complex, protein transport
• Biological source: Homo sapiens (Human); More
• Cellular location: Golgi apparatus membrane ; Lipid- anchor . Isoform 1: Golgi apparatus membrane ; Lipid-anchor . Isoform 2: Golgi apparatus membrane ; Lipid-anchor : P20340; Golgi apparatus : P97329
• Total number of polymer chains: 4
• Total formula weight: 61207.03

Authors

Bressanelli, G.,Pylypenko, O.,Houdusse, A. (deposition date: 2016-06-29, release date: 2017-11-15, Last modification date: 2024-10-16)

Primary citation

Miserey-Lenkei, S.,Bousquet, H.,Pylypenko, O.,Bardin, S.,Dimitrov, A.,Bressanelli, G.,Bonifay, R.,Fraisier, V.,Guillou, C.,Bougeret, C.,Houdusse, A.,Echard, A.,Goud, B.
Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.
Nat Commun, 8:1254-1254, 2017

PubMed Abstract: The actin and microtubule cytoskeletons play important roles in Golgi structure and function, but how they are connected remain poorly known. In this study, we investigated whether RAB6 GTPase, a Golgi-associated RAB involved in the regulation of several transport steps at the Golgi level, and two of its effectors, Myosin IIA and KIF20A participate in the coupling between actin and microtubule cytoskeleton. We have previously shown that RAB6-Myosin IIA interaction is critical for the fission of RAB6-positive transport carriers from Golgi/TGN membranes. Here we show that KIF20A is also involved in the fission process and serves to anchor RAB6 on Golgi/TGN membranes near microtubule nucleating sites. We provide evidence that the fission events occur at a limited number of hotspots sites. Our results suggest that coupling between actin and microtubule cytoskeletons driven by Myosin II and KIF20A ensures the spatial coordination between RAB6-positive vesicles fission from Golgi/TGN membranes and their exit along microtubules.

PubMed: 29093437
DOI: 10.1038/s41467-017-01266-0

Experimental method

X-RAY DIFFRACTION (2.088 Å)