5LEF

Rab6A:Kif20A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976273
Spacegroup name	P 21 21 2
Unit cell lengths	125.250, 128.190, 36.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.793 - 2.088
R-factor	0.1778
Rwork	0.176
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gil
RMSD bond length	0.003
RMSD bond angle	0.733
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.210
High resolution limit [Å]	2.088	2.088
Rmerge		0.733
Number of reflections	34406
<I/σ(I)>	14.11
Completeness [%]	96.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	Hepes 0.1M, Ammonium Sulfate 0.2M, PEG 8K 24%, Isopropanol 2%