5KKL

Structure of ctPRC2 in complex with H3K27me3 and H3K27M

Summary for 5KKL

Entry DOI	10.2210/pdb5kkl/pdb
Descriptor	putative polycomb protein Eed, Putative uncharacterized protein,Histone H3.1 peptide,Zinc finger domain-containing protein, ALA-ALA-ARG-M3L-SER-ALA-PRO-ALA, ... (5 entities in total)
Functional Keywords	complex, methyltransferase, histone h3, transferase
Biological source	Chaetomium thermophilum More
Total number of polymer chains	3
Total formula weight	174980.00
Authors	Jiao, L.,Liu, X. (deposition date: 2016-06-21, release date: 2017-04-19, Last modification date: 2023-09-27)
Primary citation	Jiao, L.,Liu, X. Response to Comment on "Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2". Science, 354:1543-1543, 2016 Cited by PubMed Abstract: Zhang et al suggested that in the crystal structure of a polycomb repressive complex 2 from Chaetomium thermophilum (ctPRC2), a flexible linker region, but not the H3K27M cancer mutant peptide, better fits the electron density. Based on our new data, we agree with this alternative interpretation and provide the crystal structure of ctPRC2 bound to a bona fide H3K27M sequence. PubMed: 28008038 DOI: 10.1126/science.aaj2335 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.94 Å)

Structure validation