5KKL
Structure of ctPRC2 in complex with H3K27me3 and H3K27M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2016-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.254, 80.559, 133.220 |
Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
Resolution | 48.740 - 2.940 |
R-factor | 0.1885 |
Rwork | 0.186 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kjh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.077 | 1.450 |
Rpim | 0.032 | |
Number of reflections | 36440 | 1739 |
<I/σ(I)> | 24.15 | 1.36 |
Completeness [%] | 98.0 | 94.7 |
Redundancy | 6.5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG4000, 175mM ammonium citrate, pH 7.0 |