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5K0Y

m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face

Summary for 5K0Y
Entry DOI10.2210/pdb5k0y/pdb
EMDB information8190
DescriptortRNA, ribosomal protein uS13, Eukaryotic translation initiation factor 3 subunit G, ... (42 entities in total)
Functional Keywordseukaryotic translation initiation, ribosome, eif3 peripheral subunits, cryo-em, translation
Biological sourceOryctolagus cuniculus (Rabbit)
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Cellular locationCytoplasm : O75821 O75821 Q5IH81 G1TN72
Total number of polymer chains42
Total formula weight1291316.63
Authors
Simonetti, A.,Brito Querido, J.,Myasnikov, A.G.,Mancera-Martinez, E.,Renaud, A.,Kuhn, L.,Hashem, Y. (deposition date: 2016-05-17, release date: 2016-07-13, Last modification date: 2018-04-18)
Primary citationSimonetti, A.,Brito Querido, J.,Myasnikov, A.G.,Mancera-Martinez, E.,Renaud, A.,Kuhn, L.,Hashem, Y.
eIF3 Peripheral Subunits Rearrangement after mRNA Binding and Start-Codon Recognition.
Mol.Cell, 63:206-217, 2016
Cited by
PubMed Abstract: mRNA translation initiation in eukaryotes requires the cooperation of a dozen eukaryotic initiation factors (eIFs) forming several complexes, which leads to mRNA attachment to the small ribosomal 40S subunit, mRNA scanning for start codon, and accommodation of initiator tRNA at the 40S P site. eIF3, composed of 13 subunits, 8 core (a, c, e, f, h, l, k, and m) and 5 peripheral (b, d, g, i, and j), plays a central role during this process. Here we report a cryo-electron microscopy structure of a mammalian 48S initiation complex at 5.8 Å resolution. It shows the relocation of subunits eIF3i and eIF3g to the 40S intersubunit face on the GTPase binding site, at a late stage in initiation. On the basis of a previous study, we demonstrate the relocation of eIF3b to the 40S intersubunit face, binding below the eIF2-Met-tRNAi(Met) ternary complex upon mRNA attachment. Our analysis reveals the deep rearrangement of eIF3 and unravels the molecular mechanism underlying eIF3 function in mRNA scanning and timing of ribosomal subunit joining.
PubMed: 27373335
DOI: 10.1016/j.molcel.2016.05.033
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (5.8 Å)
Structure validation

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