5J7C

A picomolar affinity FN3 domain in complex with hen egg-white lysozyme

Summary for 5J7C

Related5J7K
DescriptorLysozyme C, FNfn10-anti-lysozyme (DE0.4.1) (3 entities in total)
Functional Keywordsyeast surface display, high affinity, fibronectin type iii, fn3, protein binding-hydrolase complex, protein binding/hydrolase
Biological sourceHomo sapiens
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Cellular locationSecreted P00698
Total number of polymer chains4
Total molecular weight51255.64
Authors
Porebski, B.T.,Drinkwater, N.,McGowan, S.,Buckle, A.M. (deposition date: 2016-04-06, release date: 2016-08-17, Last modification date: 2016-09-21)
Primary citation
Porebski, B.T.,Conroy, P.J.,Drinkwater, N.,Schofield, P.,Vazquez-Lombardi, R.,Hunter, M.R.,Hoke, D.E.,Christ, D.,McGowan, S.,Buckle, A.M.
Circumventing the stability-function trade-off in an engineered FN3 domain.
Protein Eng.Des.Sel., 2016
PubMed: 27578887 (PDB entries with the same primary citation)
DOI: 10.1093/protein/gzw046
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.535 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.249201.1%0.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload